Introduction
The AveMix® 02 CS formulation contains three different enzymatic activities and is available in dry and liquid forms. The product is authorised for use in the EU in piglets. AveMix® 02 CS is available in solid and liquid forms.
Manufacturer´s main claims
Improves animal performance due to higher digestibility of the feed
Enables high inclusion of by-products
Technical specifications of AveMix® 02 CS (Solid and Liquid)
EU category
Zootechnical additives
Functional group of additives
Digestibility enhancers
Active substance
endo-1,4-beta-xylanase (EC 3.2.1.6)
endo-1,3(4)-beta-glucanase (EC 3.2.1.8)
polygalacturonase (pectinase) (EC 3.2.1.15)
Enzymatic activity in the product
Solid form:
endo-1,4-beta-xylanase: 21,400 XU/g
endo-1,3(4)-beta-glucanase: 12,300 BGU/g
polygalacturonase (pectinase): 460 PGLU/g
Liquid form:
endo-1,4-beta-xylanase: 10,700 XU/g
endo-1,3(4)-beta-glucanase: 6,150 BGU/g
polygalacturonase pectinase: 230 PGLU/g
EU approval, minimum content
Piglets weaned and suckling
Xylanase: 2140 XU/kg feed
Beta-glucanase: 1230 BGU/kg feed
Polygalacturonase (pectinase): 46 PGLU/kg feed
End of period of EU authorisation
5 March 2035
EU code
4a14
Production organism
endo-1,3(4)-beta-glucanase: Trichoderma reesei, (MUCL 49754)
endo-1,4-beta-xylanase: Trichoderma reesei, (MUCL 49755)
polygalacturonase: Aspergillus fijiensis, (CBS 589.94)
Product storage stability
Solid form: When stored for 12 months at 25°C, the remaining activities of xylanase, glucanase, and pectinase were 96, 98 and 97%, respectively.
Liquid form: The product retained 96, 92 and 83 % of the initial activity of xylanase, beta-glucanase and pectinase, respectively, when stored for six months at 4° C. When stored at 30°C instead of 4°C, the corresponding figures were 79, 82 and 57 %, respectively.
Storage stability in premix and feed
Solid form: When the product was incorporated into a premix containing minerals and vitamins and stored at 25°C for three months, xylanase, glucanase, and pectinase activities were 85, 94 and 88%, respectively.
No data on storage stability in feed or heat stability is available
Heat stability
Solid form: The loss of xylanase and beta-glucanase activity was 25 % when the feed was pelleted at 75°C. The activity of pectinase was not measured.
Liquid form: The liquid formulation is intended for post-pelleting application
Homogeneity
Carrier
Solid: Soybean meal
Liquid: water and sorbitol
Handling
Breathing protection, glasses and gloves shall be used during handling
Manufacturer
Palital
List of references
EFSA (2009). Scientific Opinion. Safety and efficacy of AveMix® 02 CS/L (endo-1,3(4)-β-glucanase, endo-1,4-β-xylanase and pectinase) as feed additive for weaned piglets. The EFSA Journal (2009) 1186, 1-17
EFSA (2011). Scientific Opinion on the efficacy of AveMix® 02 CS/L (endo-1,3(4)-beta-glucanase, endo-1,4-beta-xylanase and polygalacturonase) as feed additive for weaned piglets. EFSA Journal 2011;9(2):2010
EU (2025). COMMISSION IMPLEMENTING REGULATION (EU) 2025/284 of 12 February 2025 concerning the renewal of the authorisation of a preparation of endo-1,4-beta-xylanase produced with Trichoderma reesei MUCL 49755, endo-1,3(4)-beta-glucanase produced with Trichoderma reesei MUCL 49754 and polygalacturonase produced with Aspergillus fijiensis CBS 589.94 as a feed additive for weaned piglets and the authorisation of that preparation as a feed additive for suckling piglets (holder of authorisation: AVEVE BV) and repealing Implementing Regulation (EU) No 527/2011. Official Journal of the European Union. http://data.europa.eu/eli/reg_impl/2025/284/oj