Introduction
Axtra XB 201 contains beta-xylanase and beta-glucanase. The product is authorised for use in the EU in piglets, pigs for fattening, lactating sows and poultry. Axtra XB 201 is available in both dry and liquid forms.
Manufacturer´s main claims
Improves animal growth and feed conversion
Reduces feed costs
Improves animal uniformity
Dose rate is flexible
Contributes to a better environment
Technical specifications of Axtra XB 201
EU category
Zootechnical additives
Functional group of additives
Digestibility enhancers
Active substance
Endo-1,4-beta-xylanase (EC 3.2.1.8)
Endo-1,3(4)-beta-glucanase (EC 3.2.1.6)
Enzymatic activity in the product
endo-1,4-beta-xylanase: 12,200 U/g
endo-1,3(4)-beta-glucanase: 1,520 U/g
EU approval, minimum content per kg feed
1830 U xylanase and 228 U beta-glucanase
Laying hens
1220 U xylanase and 152 U beta-glucanase
Lactating sows
Minor porcine species (lactating sows)
915 U xylanase and 114 U beta-glucanase
Other poultry
Suckling piglets
Weaned piglets
Pigs for fattening
Minor porcine species (suckling piglets, weaned piglets and pigs for fattening)
End of period of EU authorisation
Poultry; turkeys for fattening and reared for breeding; laying hens: 28 April 2021*
Piglets and pigs for fattening: 28 April 2021*
*: Following the provisions of Art. 14 of Reg. (EC) No 1831/2003, an application, in accordance with Article 7, has been submitted
EU code
4a15
Production organism
Endo-1,4-beta-xylanase: Trichoderma reesei (ATCC PTA 5588)
Endo-1,3(4)-beta-glucanase: Trichoderma reesei (ATCC SD 2106)
Product storage stability
Solid form: When stored for 12 months at 25°C, there was a 4-12% activity loss. If the product was stored at 40°C for three months, 79% of the initial xylanase activity and 91 % of the initial beta-glucanase activity were recovered.
Liquid form: After 12 months of storage at 25°C, the xylanase and beta-glucanase activities were 77% and 97 % of the initial values, respectively. If stored at 40°C for three months, the recovery of xylanase was 85 % and for beta-glucanase 100%. After six months at 40°C, the xylanase activity was reduced to 72 %, and the beta-glucanase activity was 88%.
Storage stability in premix and feed
Solid form
The Axtra product was added to a premixture containing trace elements and stored for six months at either 25°C or 40°C. There was no reduction in the initial xylanase activity when stored at 25°C, whereas beta-glucanase activity was reduced by 9 %. Xylanase and beta-glucanase activity were reduced by 11 and 29 %, respectively, when stored at 40°C. The stability of Axtra, when added to mash feed, has also been investigated. After three months of storage at 25°C, the recovered xylanase and beta-glucanase activity were 87 % and 92 %, respectively. If the feed was stored at 40°C, the xylanase and beta-glucanase activity were reduced to 34 and 42 % of the initial activity, respectively. When added to pelleted feed, more than 85% of the initial activity of xylanase and beta-glucanase could be recovered after three months of storage at 25°C.
Liquid form
The recovery rate of enzymatic activity in pelleted feed was higher than 94 % when stored for three months at 25°C. When the feed was stored at 40°C for three months, the recovery rate for xylanase and beta-glucanase was higher than 56%.
Heat stability
Solid form: The average recovery of xylanase and beta-glucanase when the feed was pelleted at 90°C was 106 and 85%, respectively.
Liquid form: For post-pelleting application
Homogeneity
The solid formulation was tested for its homogeneous mixing ability in mash or pelleted feed. Ten subsamples of mash or pelleted feed supplemented with the solid form were analysed. Two different dosages were added: 50 or 250 mg/kg feed. The results showed a CV lower than 10% for the low dosage and below 6% for the high dosage.
The liquid formulation was sprayed onto pellets and analysed for its homogeneous mixing ability. Ten subsamples of the feed supplemented were analysed. Two different dosages were added: 50 or 250 mg/kg feed. The results showed a CV lower than 6% for both enzyme inclusion ratios.
Carrier
Sodium sulphate, hydrated magnesium silicate, starch, sucrose and polyvinyl alcohol
Sorbitol, sodium chloride, sodium acetate and potassium sorbate
Package size
Solid form: 25 kg bags or 1000 kg bulk bags
Liquid form: 200 or 1000 kg containers
Handling
The European Commission states: "For users of the additive and premixtures, feed business operators shall establish operational procedures and organisational measures to address the potential risks resulting from their use. Where those risks cannot be eliminated by such procedures and measures, the additive and premixtures shall be used with personal breathing, skin and eye protective equipment."
Manufacturer
IFF
List of references
EFSA (2010). Scientific opinion on the safety and efficacy of Danisco Glycosidase TPT/L (endo-1,4-beta-xylanase and endo-1,3(4)-beta-glucanase) as feed additive for poultry, piglets and pigs for fattening. EFSA Journal 2010; 8:(12):1916
EU (2011). Commission Regulation (EU) no 337/2011 of 7 April 2011 concerning the authorisation of an enzyme preparation of endo-1,4-beta-xylanase and endo-1,3(4)-beta-glucanase as feed additive for poultry, weaned piglets and pigs for fattening (holder of the authorisation Danisco Animal Nutrition). Official Journal of the European Union. L 94/19
EU (2016). Commission Regulation (EU) no 2016/997 of 16 June 2016 concerning the authorisation of endo-1,4-beta-xylanase EC 3.2.1.8 produced by Trichoderma reesei (ATCC PTA 5588) and endo-1,3(4)-beta-glucanase EC 3.2.1.6 produced by Trichoderma reesei (ATCC SD 2106) as a feed additive for lactating sows and minor porcine species (holder of authorisation Danisco (UK) Ltd)
EU (2023) COMMISSION IMPLEMENTING REGULATION (EU) 2023/1703 of 7 September 2023 concerning the renewal of the authorisation of a preparation of endo-1,4-beta-xylanase produced by Trichoderma reesei CBS 143953 and endo-1,3(4)-beta-glucanase produced by Trichoderma reesei CBS 143945 as a feed additive for poultry species, weaned piglets, pigs for fattening, lactating sows and minor porcine species (weaned piglets, pigs for fattening and lactating sows), the authorisation of that preparation for suckling piglets and minor porcine species (suckling piglets) (holder of authorisation: Danisco (UK) Ltd, trading as Danisco Animal Nutrition and represented by Genencor International B.V.) and repealing Regulation (EU) No 337/