Introduction
Bacillus amyloliquefaciens (DSM 25840) is a single-strain probiotic authorised for pigs in the EU. The DSM 25840 Bacillus amyloliquefaciens is used as a component in the SolPreme® product.
Technical specifications of Bacillus amyloliquefaciens DSM 25840
EU category
Zootechnical additives
Functional group of additives
Gut flora stabilisers
Active substance
Bacillus amyloliquefaciens (DSM 25840)
Activity in the product
Activity stated in the EU authorisation: 1.25 x 1010 CFU/g
EU approval, minimum content
All porcine species.
5 x 108 CFU/kg feed or 1.7 x 108 CFU/l of drinking water
End of period of authorisation
21 October 2030
EU code
4b1901
Recommended dosage
Minimum dosage authorised in EU: For all the above species, a minimum of each strain: 0.5 x 108 CFU/kg feed or 1.7 x 108 CFU/l drinking water
Product storage stability
Three batches of the product were used to test the stability of the product when stored at 25, 30 or 37°C for one year. Storage at these temperatures had a negligible effect on viable spores (<0.5 log).
Storage stability in feed, premix and water
Two vitamin-mineral premixes were supplemented with the product and stored for six months at 20-25°C. No reductions in viable spores could be detected. The stability of the product, when added to feed and stored for six months at 20-25°C, was also tested. No losses could be detected. Samples of the product from three different product batches were dissolved in drinking water. The water was stored at 20-25°C and analysed after one and two days. More than 90% of the added spores could be detected at both time points.
Heat stability
The product´s heat stability was studied using three different pelleting temperatures: 75, 85 and 95°C. The rate of recovery was 95% at all temperatures.
Compatibility
Compatible with diclazuril, decoquinate and halofuginone.
Physical properties
Laser diffraction was used to determine the particle size distribution. Eight per cent of the particles were smaller than 50 µm, and 4 % were smaller than 10 µm. A dusting potential test showed that the product has a dusting potential of 13.5 g/m3
Ten subsamples of a batch of pelleted feed with the product were analysed to test the capacity to mix homogeneously. The analytical results showed a CV of 0.4%.
Handling
The European Commission states: "For users of the additive and premixtures, feed business operators shall establish operational procedures and organisational measures to address potential risks resulting from its use. Where those risks cannot be eliminated or reduced to a minimum by such procedures and measures, the additive and premixtures shall be used with personal protective equipment, including skin, eyes and breathing protection."
Producer
Chr. Hansen
List of references
EFSA (2018). Safety and efficacy of ZM16 10 (Bacillus amyloliquefaciens DSM 25840) as a feed additive for weaned piglets and minor porcine species. EFSA Journal 2018;16(4):5200
EFSA (2021). Safety and efficacy of an additive consisting of Bacillus amyloliquefaciens DSM 25840 for all animal species (Chr. Hansen A/S). EFSA Journal 2021;19(4):6522