Enviva Pro

Introduction  
Enviva® PRO is a probiotic product containing three strains of Bacillus velenzensis. This bacillus was formerly known as Bacillus amyloliquefaciens. The product is authorised in the EU for use in poultry.

The manufacturer´s main claims
Improved cost per kg gain
Improved caloric efficiency
Proven value in antibiotic growth promoter-free nutrition
Contributing to food safety

Technical specifications of Enviva PRO®

EU category
Zootechnical additive

Functional group of additives
Gut flora stabiliser

Active substance
Bacillus velezensis (PTA-6507), formerly known as Bacillus amyloliquefaciens, PTA-6507
Bacillus velezensis (NRRL B-50013) formerly known as Bacillus amyloliquefaciens, NRRL B-50013
Bacillus velezensis (NRRL B-50104) formerly known as Bacillus amyloliquefaciens, NRRL B-50104 

Activity in the product
Minimum content:
2.5 x 109 CFU/g
The product contains a minimum of 8.3 x 108 CFU/g of each strain

EU approval
The minimum dosage for the below species: 7.5 x 107 CFU/kg feed
Chickens for fattening
Chickens reared for laying
Minor poultry species for fattening or reared for laying
Turkeys for fattening 

End of period of authorisation
Chickens for fattening: 3 April 2027
Chickens reared for laying: 3 April 2027
Minor poultry species for fattening or reared for laying: 3 April 2027
Turkeys for fattening: 15 December 2031

EU code
4b1827i

Product storage stability
Three batches of the product were stored in sealed bags for 24 months at either 22°C or 37°C. After 24 months of storage, the loss of Bacilli was less than 0.5 log values.
Storage stability in feed and premix    

Storage stability in feed and premix
Three different batches of the product were supplemented to a vitamin/mineral premix. Samples of the premix were stored for six months at either 22°C or 37°C in sealed foil bags. The loss of bacterial count was less than 0.5 log values after six months. 
Storage of pelleted feed supplemented with the product for three months did not affect the number of viable counts. Similar studies with mash feed confirmed no losses of viable counts after three months of storage.

Heat stability
Mash feed supplemented with the product was pelleted for 30 seconds at 95°C. No loss of viable counts was detected. 

Homogeneity    
The sub-samples of a mash poultry feed were used to study the capacity of the product to distribute homogeneously in the feed. The coefficient of variance found in the sub-samples was 3%.

Physical properties
The fraction of particles below 100 μm was about 2 %. The mean dusting potential is 0.48 g/m3. 

Compatibility claims    
The product is compatible with narasin/nicarbazin, maduramicin ammonium, lasalocid A sodium, salinomycin sodium, monensin sodium, robenidine hydrochloride, diclazuril, decoquinate, semduramycin sodium or nicarbazin.

Handling
Inhalation of dust should be avoided. Gloves and goggles should be worn to reduce contact with dust.

Producer
Iff

List of references

EFSA (2016).  Safety and efficacy of Enviva PRO 202 GT (Bacillus amyloliquefaciens PTA-6507, Bacillus amyloliquefaciens NRRL B-50013 and Bacillus amyloliquefaciens NRRL B-50104) for chickens for fattening, chickens reared for laying and minor poultry species for fattening and to point of lay. EFSA Journal 2016;14(6):4505

EU (2017). Commission Implementing Regulation (EU) 2017/440 of 13 March 2017 concerning the authorisation of the preparation Bacillus amyloliquefaciens (PTA-6507), Bacillus amyloliquefaciens (NRRL B-50013) and Bacillus amyloliquefaciens (NRRL B-50104) as a feed additive for chickens for fattening, chickens reared for laying, minor poultry species for fattening and minor poultry species reared for laying (holder of authorisation Danisco (UK) Ltd, trading as Danisco Animal Nutrition). Official Journal of the European Union. L 67/74-77

EU (2021). Commission Implementing Regulation (EU) 2021/2051 of 24 November 2021 concerning the authorisation of a preparation of Bacillus velezensis PTA-6507, Bacillus velezensis NRRL B-50013 and Bacillus velezensis NRRL B-50104 as a feed additive for turkeys for fattening (holder of authorisation: Danisco Animal Nutrition represented by Genencor International B.V.). Official Journal of the European Union. L 420/19-22