Introduction
Lactobacillus acidophilus D2/CSL is a product intended for use in feed or drinking water in several avian species. The product is also approved for use in cat and dog food.
Manufacturer´s main claims
Prevents dysbiosis
Improves digestive functions
Increases overall performance
Improves egg quality
Technical specifications of Lactobacillus acidophilus D2/CSL
EU category
Zootechnical additives
Functional group of additives
Gut flora stabilisers
Active substance
Lactobacillus acidophilus (CECT 4529)
Activity in the product
Lactobacillus acidophilus: 5 x 1010 CFU/gram
EU approval, minimum content per kg feed
No maximum inclusion for the animal categories below
All poultry species and categories except laying hens and chickens for fattening: 1 x 109 CFU/kg feed
All poultry species and categories except laying hens and chickens for fattening: 5 x 108 CFU/litre drinking water
Ornamental birds: 1 x 109 CFU/kg feed
Ornamental birds: 5 x 108 CFU/litre drinking water
Laying hens: 1 x 109 CFU/kg feed
Laying hens: 5 x 108 CFU/l of drinking water
Chickens for fattening: 1 x 109 CFU/kg feed
Chickens for fattening: 5 x 108 CFU/litre drinking water
Cats and dogs: 5 x 109 CFU/kg feed
End of period of authorisation
All poultry species and categories except laying hens and chickens for fattening: 12 September 2032
Ornamental birds: 12 September 2032
Laying hens: 3 March 2025*
Chickens for fattening (feed): 29 December 2027
Chickens for fattening (water): 12 September 2032
Cats and dogs: 7 November 2028
*: Following the provisions of Art. 14 of Reg (EC) 1831/2003, an application, in accordance with Art. 7, has been submitted.
EU code
4b1715
Product storage stability
Product stability was tested at various temperatures. At temperatures between 2 and 8°C, the product was stable for 14 months with an 8 % loss of activity. After six months, when stored at 20°C, there was an activity loss of 20%. At a storage temperature of 40°C, the activity was reduced considerably after two weeks.
Storage stability in feed, premix and tap water
The product was added to a premix and complete feed and stored at 20°C. After one month in the premix and the feed, a reduction of 0.5 log values was observed. After 13 weeks of feed storage, the activity in the feed was reduced by 2 log values. At 4°C, tap water product activity was good after 24 hours. When the water temperature was 20°C, a loss of more than 0.5 log values was observed.
Heat stability
Recommended only for mash feed
Carrier
Lactose
Package size
44 g aluminium polythene sachets
Handling
During handling, breathing protection shall be used
Producer
Sacco System
List of references
EFSA (2014). Scientific Opinion on the Safety and Efficacy of Lactobacillus acidophilus D2/CSL (Lactobacillus acidophilus) for laying hens. EFSA Journal 2014;12(7):3789
EFSA (2017). Safety and efficacy of Lactobacillus acidophilus D2/CSL (Lactobacillus acidophilus CECT 4529) as a feed additive for chickens for fattening. EFSA Journal 2017;15(4):4762
EU (2015). Commission Implementing Regulation (EU) No 2015/38 of 13 January 2015 concerning the authorisation of the preparation of Lactobacillus acidophilus CECT 4529 as a feed additive for laying hens and amending Regulation (EC) No 1520/2007 (holder of authorisation Centro Sperimentale del Latte). Official Journal of the European Union. L 8/4-6
EU (2017). Commission Implementing Regulation (EU) No 2017/2275 of 8 December 2017 concerning the authorisation of a new use of the preparation of Lactobacillus acidophilus (CECT 4529) as a feed additive for chickens for fattening (holder of the authorisation Centro Sperimentale del Latte). Official Journal of the European Union. L326/47-49
EU (2018). Commission Implementing Regulation (EU) 2018/1558 of 17 October 2018 concerning the authorisation of a new use of the preparation of Lactobacillus acidophilus (CECT 4529) as a feed additive for cats and dogs (holder of authorisation Centro Sperimentale del Latte). Official Journal of the European Union L 261/13-15
EU (2022). Commission Implementing Regulation (EU) 2022/1417 of 22 August 2022 concerning the authorisation of a preparation of Lactobacillus acidophilus CECT 4529 as a feed additive for all poultry species and categories, other than laying hens and chickens for fattening, and for ornamental birds and amending Implementing Regulation (EU) 2017/2275 (holder of authorisation: Centro Sperimentale del Latte S.r.l.). Official Journal of the European Union. L 218/3-6