Introduction
The Ronozyme® HiPhos product range is the predecessor to the HiPhorius phytase from the Novozymes DSM alliance. The EFSA scientific opinion was published in June 2012.
Ronozyme® HiPhos is available both in dry and liquid forms. The Ronozyme HiPhos products are available in different concentrations.
Ronozyme® HiPhos products:
Ronozyme® HiPhos (GT) (Granulates) for pelleted feed
Ronozyme® HiPhos (L) (Aqueous liquid) for post-pelleting application
Manufacturer´s main claims
Increases the amount of plant phosphorus available to the animal
Substantial savings on feed costs
Technical specifications of Ronozyme® HiPhos (GT) and (L)
EU category
Zootechnical additives
Functional group of additives
Digestibility enhancers
Active substance
6-phytase (EC 3.1.3.26)
Phytase activity in the product
Available in different concentrations starting with 10,000 FYT/g
EU approval, minimum content
500 FYT/kg feed for the animal categories below:
Poultry
Piglets (weaned)
Pigs for fattening
Sows
End of period of EU authorisation
23 July 2033
EU code
4a18i
Production organism
Aspergillus oryzae (DSM 33699)
Donor organism
Citrobacter braakii
Product storage stability
GT: Retains more than 90 % activity when stored for 18 months at 25 °C
L: When kept in a closed container at 25°C for 12 months, the product retained more than 80 % activity.
Storage stability in premix and feed
GT: In compound feed, 84 to 90 % of the initial activity was retained when the feed was stored at 25 °C for three months.
L: In pelleted feed, more than 78 % of the initial activity was retained when the feed was stored at 25 °C
Heat stability
GT: More than 100 % of the phytase activity was left when the feed was pelleted at 80 °C. Higher pelleting temperatures at 90°C showed a loss of activity of 6 %.
L: The product is intended to be used post-pelleting.
Homogeneity
Subsamples of batches of pelleted feed with the product were analysed to test the capacity to mix homogeneously. The analytical results showed a CV that ranged from 8.7 to 10%
Physical properties
Laser diffraction was used to determine the particle size distribution. The results showed that the particle size ranged from 180 to 500 µm. A dusting potential test showed that the product has an average dusting potential of 9.9 mg/m3.
Carrier
Sodium sulphate, cellulose and dextrin
Handling
The European Commission states: "For users of the additive and premixtures, feed business operators shall establish operational procedures and organisational measures to address potential risks resulting from its use. Where those risks cannot be eliminated by such procedures and measures, the additive and premixtures shall be used with personal breathing, eye and skin protective equipment."
List of references
EFSA (2012). Scientific opinion on Safety and efficacy of Ronozyme HiPhos GT (6-phytase) as feed additive for poultry and pigs. EFSA Journal 2012; 10(6): 2730
EFSA (2012). Scientific Opinion on the Safety and efficacy of Ronozyme HiPhos M/L (6-phytase) as a feed additive for poultry and pigs. EFSA Journal 2012; 10(1): 2527
EFSA (2023). Safety and efficacy of the feed additive consisting of 6-phytase (produced by Aspergillus oryzae DSM 33699) (RONOZYME® Hiphos GT/L) for poultry, pigs for fattening,
weaned piglets and sows (DSM Nutritional Products Ltd). EFSA Journal 2023; 21(1):7698
EU (2012). Commission Implementing Regulation (EU) No 837/2012 of 18 September 2012 concerning the authorisation of 6-phytase (EC 3.1.3.26) produced by Aspergillus oryzae (DSM 22594) as feed additive for poultry, weaned piglets, pigs for fattening and sows (holder of the authorisation DSM Nutritional Products). Official Journal of the European Union, L 252/7
EU (2012). Commission Implementing Regulation (EU) No 1265/2012 of 17 December 2012 amending Implementing Regulation (EU) No 837/2012 as regards the minimum activity of a preparation of 6-phytase produced by Aspergillus oryzae (DSM 22594) as feed additive for poultry, weaned piglets, pigs for fattening and sows (holder of authorisation DSM Nutritional Products). Official Journal of the European Union L 356/61
EU (2016). Commission Implementing Regulation (EU) No 2016/1881 of 24 October 2016 amending Regulation (EU) No 837/2012 as regards the minimum activity of 6-phytase produced by Aspergillus oryzae (DSM 22594) as feed additive for sows (holder of authorisation DSM Nutritional Products Ltd). Official Journal of the European Union. L 289/15-17
EU (2023). COMMISSION IMPLEMENTING REGULATION (EU) 2023/1342 of 30 June 2023 concerning the renewal of the authorisation of a preparation of 6-phytase produced by Aspergillus oryzae DSM 33699 as a feed additive for poultry, pigs for fattening, weaned piglets and sows (holder of authorisation: DSM Nutritional Products Ltd, represented by DSM Nutritional Products Sp. z o.o.) and repealing Implementing Regulation (EU) No 837/2012. Official Journal of the European Union. L168/17-21