Introduction
Ronozyme® VP is a product containing beta-glucanase and various other side-activities such as pentosanase, hemicellulase and enzymes which can hydrolyse pectic substances. The product is authorised for use in the EU in piglets and chickens for fattening. Ronozyme® VP is available in dry and liquid forms: Ronozyme® VP CT (granular) and Ronozyme® VP L (liquid)
Manufacturer´s main claims
Improves the digestibility of vegetable protein
Increase daily weight gain and feed conversion
Technical specifications of Ronozyme® VP CT (granular) and L (liquid)
EU category
Zootechnical additives
Functional group of additives
Digestibility enhancers
Active substance
endo-1,3(4)-beta-glucanase (EC 3.2.1.6)
Enzymatic activity in the product
Solid product: 50 FBG/g
Liquid product: 120 FBG/ml
EU approval, minimum content
Piglets: 10 FBG/kg feed
Chickens for fattening: 10 FBG/kg feed
End of period of EU authorisation
20 July 2033
EU code
4a1603
Production organism
Aspergillus fijiensis (CBS 589.94)
Product storage stability
Three batches of each formulation were stored in commercial packaging for 104 weeks at 10°C or 25°C. Samples were also stored at 40°C for up to 13 weeks. The solid formulation lost below 10% of the initial enzyme activity at all storage conditions. The liquid formulation lost less than 10% of the initial activity when stored at 10 or 25°C for 52 weeks. After 104 weeks, only 80% of the initial activity remained. At 40°C, the loss of enzyme activity was 20% after 13 weeks.
Storage stability in premix and feed
Three different batches of the solid formulation were added to two different vitamin/mineral premixes with and without choline chloride. Samples of the premix were stored for six months at 25°C. After six months of storage, the remaining enzyme activity was 98% in the premix with choline chloride and 73% without.
The stability of the solid enzyme was studied in mash feed and pelleted feed. Feed samples were stored for three months at 25°C or 35°C. The remaining enzyme activity was 85% in the pelleted feed and 96% in the meal feed stored at 25°C. At 35°C, 30% and 19% of the initial enzyme activity was lost in the meal and pelleted feed.
The stability of the liquid formulation was tested in pelleted feed. Samples of this feed were stored for up to 13 weeks at 25°C or 35°C. The enzyme loss was 19% at 25°C and 25% at 35°C.
Heat stability
The solid formulation's heat stability was tested at 75°C. At this pelleting temperature, no loss of enzymatic activity was observed.
Physical properties
A dusting potential test showed that the solid formulation dusting potential ranged from 8 to 24 mg/m3.
Laser diffraction was used to determine the particle size distribution. The test showed that the mean particle size was 540 µm in diameter. Less than 0.5% of the particles were smaller than 250.
Homogeneity
The solid formulation was tested for its homogeneous mixing ability in mash or pelleted feed. Eight subsamples of each feed supplemented with the solid forms were analysed. The Coefficient of Variance was 13% in the mash feed and 19% in the pelleted feed.
Carrier
Solid formulation: enzyme concentrate, dextrin, kaolin, cellulose, palm oil, calcium carbonate, sodium sulphate and water
Liquid formulation: enzyme concentrate, sucrose, potassium sorbate, sodium chloride and water
Handling
The European Commission states: "For users of the additive and premixtures, feed business operators shall establish operational procedures and organisational measures to address potential risks resulting from their use. Where those risks cannot be eliminated by such procedures and measures, the additive and premixtures shall be used with personal breathing, eye and skin protective equipment."
List of references
EFSA (2023). Safety and efficacy of a feed additive consisting of endo-1,3 (4)-beta-glucanase produced by Aspergillus fijiensis CBS 589.94 (RONOZYME® VP (CT/L)) for chickens for fattening
and weaned piglets (DSM Nutritional Products AG). EFSA Journal 2023;21(1):7703
EU (2004). Commission Regulation (EC) No 1259/2004 of 8 July 2004 concerning the permanent authorisation of certain additives already authorised in feedingstuffs. Official Journal of the European Union. L 239/8-15
EU (2005). Commission Regulation (EC) No 1811/2005 of 4 November 2005 concerning the provisional and permanent authorisation of certain additives in feedingstuffs and the provisional authorisation of a new use of an additive already authorised in feedingstuffs. Official Journal of the European Union L291/12
EU (2023). COMMISSION IMPLEMENTING REGULATION (EU) 2023/1333 of 29 June 2023 concerning the authorisation of a preparation of endo-1,3(4)-beta-glucanase produced by Aspergillus fijiensis CBS 589.94 as a feed additive for chickens for fattening and weaned piglets (holder of authorisation: DSM Nutritional Products Ltd, represented by DSM Nutritional Products Sp. z o.o.), amending Regulation (EC) No 1811/2005 and repealing Regulation (EC) No 1259/2004. Official Journal of the European Union. L166/106-110