Kemzyme® PLUS Dry contains five different enzymatic activities. The product is authorised for use in the EU in piglets, chickens for fattening and turkeys for fattening

Manufacturer´s main claims
Provides all needed enzymatic activities for the break-down of complex substrates
Increases the availability of energy and amino acids

Table 1. Technical specifications Kemzyme® PLUS Dry

EU category Zoo-technical additive
Functional group of additives Digestibility enhancer
Active substance endo-1,3(4)-beta-glucanase (EC 3.2.1.6)
endo-1,4-beta-glucanase (EC 3.2.1.4)
alpha-amylase (EC 3.2.1.1)
bacillolysin (EC 3.4.24.28)
endo-1,4-beta-xylanase (EC 3.2.1.8)
Enzymatic activity in the product 2350 U/g endo-1,3(4)-beta-glucanase
18 000 U/g endo-1,4-beta-glucanase
400 U/g alpha-amylase
1700 U/g bacillolysin
35,000 U/g endo-1,4-beta-xylanase
EU approval, minimum content Chickens for fattening
Chickens reared for laying
Minor avian species for laying
Ornamental birds
Piglets (weaned):

endo-1,3(4)-beta-glucanase: 1175 U/kg feed
endo-1,4-beta-glucanase: 9000 U/kg feed
alpha-amylase: 200 U/kg feed
bacillolysin: 850 U/kg feed
endo-1,4-beta-xylanase: 17500 U/kg feed

All turkeys
Laying hens
Minor avian species for fattening:

endo-1,3(4)-beta-glucanase: 588 U/kg feed
endo-1,4-beta-glucanase: 4500 U/kg feed
alpha-amylase: 100 U/kg feed
bacillolysin: 425 U/kg feed
endo-1,4-beta-xylanase: 8750 U/kg feed
End of period of EU authorisation 28 June 2027
EU code 4a1620i
Derived from microorganism Aspergillus aculeatinus (CBS 589.94): endo-1,3(4)-beta-glucanase
Trichoderma reesei (CBS 592.94): endo-1,4-beta-glucanase
Bacillus amyloliquefaciens (DSM 9553): alpha-amylase
Bacillus amyloliquefaciens (DSM 9554): bacillolysin
Trichoderma viride (NIBH FERM BP 4842): endo-1,4-beta-xylanase
Product storage stability The product was stored for 12 months at two different temperatures; 22°C, 37-40°C
Remaining enzymatic activity (12 months and 22°C)
glucanase: >75%
cellulase (endo-1,4-beta-glucanase): 100%
amylase: >76%
bacillolysin: >80%
xylanase: >80%
Remaining enzymatic activity (12 months and 37-40°C)
glucanase: >75%
cellulase (endo-1,4-beta-glucanase): >96%
amylase: >57%
bacillolysin: >79%
xylanase: >58%
Storage stability in feed The product was added to a vitamin-mineral premix for sows and to mash feed based on wheat, maize and soya beans. Samples of the premix were stored for six months and samples of the mash feed were stored for three months at room temperature. Enzyme activities were stable both in the premix as well as in the feed.
Heat stability Heat stability was tested in chicken feed pelleted at 60-70°C and 70-80°C. At the low pelleting temperature enzymatic activity ranged from 82 to 94 %. The results for the highest pelleting temperatures ranged from 80 to 95% remaining enzymatic activity. 
Carrier Calcium carbonate, bentonite, fractions of yeast and vegetable oil 
Package size 25 kg paper bags or 500 kg or 1000 kg big bags
Handling Avoid ingestion, inhalation of dust or direct contact by applying suitable protective measures.
Producer Kemin

List of references

EFSA (2015). Scientific Opinion on the safety and efficacy of Kemzyme® Plus Dry (endo-1,3(4)-beta-glucanase, endo-1,4-beta-glucanase, alpha-amylase, bacillolysin and endo-1,4-beta-xylanase) for poultry, ornamental birds and piglets (weaned). EFSA Journal 2015;13(9):4234 

EU (2017). Commission Implementing Regulation (EU) 2017/963 of 7 June 2017 concerning the authorisation of the preparation of endo-1,3(4)-beta-glucanase produced by Aspergillus aculeatinus (formerly classified as Aspergillus aucleatus) (CBS 589.94), endo-1,4-beta-glucanase produced by Trichoderma reesei (formerly classified as Trichoderma longibrachiatum) (CBS 592.94), alpha-amylase produced by Bacillus amyloliquefaciens (DSM 9553), endo-1,4-beta-xylanase produced by Trichoderma viride (NIBH FERM BP4842) and bacillolysin produced by Bacillus amyloliquefaciens (DSM 9554) as a feed additive for all avian species and weaned piglets and amending Regulations (EC) No 358/2005 and (EU) No 1270/2009 (holder of the authorisation Kemin Europa NV). Official Journal of the European Union. L 145/18- 25