The product Endofeed DC contains beta-glucanase and beta-xylanase. The product is authorised for use in the EU in chickens for fattening, laying hens, pigs for fattening, minor poultry and porcine species.

Manufacturer´s main claims
Increases digestibility of nutrients
Improves daily weight gain and feed conversion
Reduces stress at weaning and diarrhoea

Table 1. Technical specifications endofeed DC

EU category Zootechnical additive
Functional group of additives Digestibility enhancer
Active substance Endo-1,3(4)-beta-glucanase (EC 3.2.1.6)
Endo-1,4-beta-xylanase (EC 3.2.1.8)
Enzymatic activity in the product 1100 U/g endo-1,3(4)-beta-glucanase
1600 U/g endo-1,4-beta-xylanase
EU approval Minimum content for species below:
Endo-1,3(4)-beta-glucanase: 138 U/kg feed
Endo-1,4-beta-xylanase: 200 U/kg feed

Chickens for fattening
Laying hens
Pigs for fattening
Minor poultry species
Minor porcine species for fattening 
End of period of EU authorisation 7 November 2027
EU code 4a1601
Derived from microorganism Both enzymes produced by Aspergillus niger (NRRL 25541)
Product storage stability Three batches of the product were stored for 18 months at either 25°C /60% RH or for six months at 40°C /75% RH. At 25°C, the residual activity of glucanase was 91% and for xylanase, 75% of the activity could be recovered. When stored at 40°C 25% of the enzymatic activity was lost after three months of storage. After six months at the high temperature, the residual enzymatic activity was below 35%.
Storage stability in premixes and feed The product was added to premix and tested at the same temperatures and humidities as in the stability test of the product itself. After six months of storage at 25°C 113% of the glucanase and 107% of the xylanase was recovered. At 40°C, the rate of recovery for glucanase and xylanase was 40 and 44 %, respectively.
Pelleted piglet feed stored for three months at an unspecified temperature and humidity lost 20% of the glucanase activity whereas the xylanase activity was unaffected by storage.
Samples of mash feed for poultry supplemented with the product was also stored at 25°C/60% RH or 40°C/75% RH. At 25°C, at least 84% of the glucanase activity and at least 90% of the xylanase could be recovered. Analyses of the feed samples kept at 40°C showed a decrease in the glucanase to lower than 52% and lower than 60% for the xylanase. 
Heat stability A mash feed for pigs based on barley, wheat and oats was added with the product and pelleted at 65-70°C. After pelleting, the glucanase activity was reduced to 74 - 85% of the initial value and for xylanase to 85-88%.
The effect of pelleting piglet feed at 50-55°C was also tested. After pelleting at the low temperatures more than 80% of the initial enzymatic activity could be recovered. 

The heat stability of the product was also tested in poultry feed pelleted at 65-70°C. The enzymatic activity after pelleting was reduced to 30% of that observed in the mash feed.
Package size 20 kg bags
Homogeneity The capacity of the product to be mixed homogeneously in mash or pelleted feed was tested in 12 sub-samples of three diets supplemented with three different levels. In mash feed, the coefficient of variance ranged from 12 to 17% for glucanase and 7 to 12 % for xylanase. The corresponding test values for pelleted feed were 11-12% for glucanase and 5 to 14% for xylanase.   
Handling Avoid inhalation of dust in accordance with standard feed manufacturing practices.
Producer Andrés Pintaluba

List of references

EFSA (2013). Scientific opinion on the safety and efficacy of Endofeed® DC (endo-1,3(4)-beta-glucanase and endo-1,4-beta-xylanases) as a feed additive for chickens for fattening, laying hens, pigs for fattening and minor poultry and porcine species. EFSA Journal 2013;11(8):3322

EU (2017). Commission Implementing Regulation (EU) 2017/1896 of 17 October 2017 concerning the authorisation of a preparation o endo-1,3(4)-beta-glucanase (EC 3.2.1.6) and endo-1,4-beta-xylanase (EC 3.2.1.8) produced by Aspergillus niger (NRRL 25541) as a feed additive for chickens for fattening, laying hens, pigs for fattening, minor poultry species and minor porcine species for fattening and amending Regulation (EC) No 255/2005 and repealing Regulation (EC) No 668/2003 (holder of the authorisation Andrés Pinaluba S.A.). Official Journal of the European Union. L 267/1-5