The product Endofeed DC contains beta-glucanase and beta-xylanase. The product is authorised for use in the EU in chickens for fattening, laying hens, pigs for fattening, minor poultry and porcine species.
Manufacturer´s main claims
Increases digestibility of nutrients
Improves daily weight gain and feed conversion
Reduces stress at weaning and diarrhoea
Table 1. Technical specifications endofeed DC
|EU category||Zootechnical additive|
|Functional group of additives||Digestibility enhancer|
|Active substance||Endo-1,3(4)-beta-glucanase (EC 188.8.131.52)
Endo-1,4-beta-xylanase (EC 184.108.40.206)
|Enzymatic activity in the product||1100 U/g endo-1,3(4)-beta-glucanase
1600 U/g endo-1,4-beta-xylanase
|EU approval||Minimum content for species below:
Endo-1,3(4)-beta-glucanase: 138 U/kg feed
Endo-1,4-beta-xylanase: 200 U/kg feed
Chickens for fattening
Pigs for fattening
Minor poultry species
Minor porcine species for fattening
|End of period of EU authorisation||7 November 2027|
|Derived from microorganism||Both enzymes produced by Aspergillus niger (NRRL 25541)
|Product storage stability||Three batches of the product were stored for 18 months at either 25°C /60% RH or for six months at 40°C /75% RH. At 25°C, the residual activity of glucanase was 91% and for xylanase, 75% of the activity could be recovered. When stored at 40°C 25% of the enzymatic activity was lost after three months of storage. After six months at the high temperature, the residual enzymatic activity was below 35%.|
|Storage stability in premixes and feed||The product was added to premix and tested at the same temperatures and humidities as in the stability test of the product itself. After six months of storage at 25°C 113% of the glucanase and 107% of the xylanase was recovered. At 40°C, the rate of recovery for glucanase and xylanase was 40 and 44 %, respectively.
Pelleted piglet feed stored for three months at an unspecified temperature and humidity lost 20% of the glucanase activity whereas the xylanase activity was unaffected by storage.
Samples of mash feed for poultry supplemented with the product was also stored at 25°C/60% RH or 40°C/75% RH. At 25°C, at least 84% of the glucanase activity and at least 90% of the xylanase could be recovered. Analyses of the feed samples kept at 40°C showed a decrease in the glucanase to lower than 52% and lower than 60% for the xylanase.
|Heat stability||A mash feed for pigs based on barley, wheat and oats was added with the product and pelleted at 65-70°C. After pelleting, the glucanase activity was reduced to 74 - 85% of the initial value and for xylanase to 85-88%.
The effect of pelleting piglet feed at 50-55°C was also tested. After pelleting at the low temperatures more than 80% of the initial enzymatic activity could be recovered.
The heat stability of the product was also tested in poultry feed pelleted at 65-70°C. The enzymatic activity after pelleting was reduced to 30% of that observed in the mash feed.
|Package size||20 kg bags|
|Homogeneity||The capacity of the product to be mixed homogeneously in mash or pelleted feed was tested in 12 sub-samples of three diets supplemented with three different levels. In mash feed, the coefficient of variance ranged from 12 to 17% for glucanase and 7 to 12 % for xylanase. The corresponding test values for pelleted feed were 11-12% for glucanase and 5 to 14% for xylanase.|
|Handling||Avoid inhalation of dust in accordance with standard feed manufacturing practices.|
List of references
EFSA (2013). Scientific opinion on the safety and efficacy of Endofeed® DC (endo-1,3(4)-beta-glucanase and endo-1,4-beta-xylanases) as a feed additive for chickens for fattening, laying hens, pigs for fattening and minor poultry and porcine species. EFSA Journal 2013;11(8):3322
EU (2017). Commission Implementing Regulation (EU) 2017/1896 of 17 October 2017 concerning the authorisation of a preparation o endo-1,3(4)-beta-glucanase (EC 220.127.116.11) and endo-1,4-beta-xylanase (EC 18.104.22.168) produced by Aspergillus niger (NRRL 25541) as a feed additive for chickens for fattening, laying hens, pigs for fattening, minor poultry species and minor porcine species for fattening and amending Regulation (EC) No 255/2005 and repealing Regulation (EC) No 668/2003 (holder of the authorisation Andrés Pinaluba S.A.). Official Journal of the European Union. L 267/1-5