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Roxazyme® G2 contains two different beta-glucanases and one xylanase. The product is authorised for use in the EU in piglets and various avian species.

Roxazyme® G2 is available in various concentrations and in dry and liquid forms:

Manufacturer´s main claims
Improves the utilization of energy in cereals and cereal by-products
Improves piglet performance

Table 1. Technical specifications Roxazyme® G2 (granular)

EU category Zoo-technical additives
Functional group of additives Digestibility enhancer
Active substance endo-1,4-beta-xylanase (EC 3.2.1.8)
endo-1,3(4)-beta-glucanase (EC 3.2.1.6)
endo-1,4-beta-glucanase (EC 3.2.1.4)
Enzymatic activity in the product 2,700 U/g endo-1,4-beta-xylanase
700 U/g endo-1,3(4)-beta-glucanase
800 U/g endo-1,4-beta-glucanase
EU approval, minimum content Piglets 
endo-1,4-beta-xylanase: 270 U/kg feed 
endo-1,3(4)-beta-glucanase: 70 U/kg feed

endo-1,4-beta-glucanase: 80 U/kg feed 
Poultry for fattening other than turkeys for fattening 
endo-1,4-beta-xylanase: 135 U/kg feed 
endo-1,3(4)-beta-glucanase: 35 U/kg feed
endo-1,4-beta-glucanase: 40 U/kg feed

Turkeys for fattening
endo-1,4-beta-xylanase: 135 U/kg feed 
endo-1,3(4)-beta-glucanase: 35 U/kg feed 
endo-1,4-beta-glucanase: 40 U/kg feed

Poultry for laying 
endo-1,4-beta-xylanase: 216 U/kg feed 
endo-1,3(4)-beta-glucanase: 56 U/kg feed 
endo-1,4-beta-glucanase: 64 U/kg feed
End of period of EU authorisation 23 May 2023
EU code 4a1602i
Derived from microorganism Trichoderma reesei (ATCC 74444)
Product storage stability In the unopened original container the product may be stored for 36 months at a temperature below 15°C.
Storage stability in feed According to the manufacturer Roxazyme G2 retains 98% of the initial enzymatic activity when mixed into a vitamine premix. The manufacturer claims that Roxazyme G2 retains 84% of the initial activity after three months when mixed into piglet mash feed
Heat stability According to the manufacturer Roxazyme G2 (G) can withstand pelleting temperatures up to 80°C. More than 80% of the initial enzymatic activity is retained at a pelleting temperature of 80°C
Package size 20 kg paper sacks
Handling Breathing protection and gloves shall be used during handling
Producer DSM

Table 2. Technical specifications Roxazyme® G2 (liquid)

EU category Zoo-technical additive
Functional group of additives Digestibility enhancer
Active substance endo-1,4-beta-xylanase (EC 3.2.1.8)
endo-1,3(4)-beta-glucanase (EC 3.2.1.6)
endo-1,4-beta-glucanase (EC 3.2.1.4)
Enzymatic activity in the product 2,700 U/ml endo-1,4-beta-xylanase
700 U/ml endo-1,3(4)-beta-glucanase
800 U/ml endo-1,4-beta-glucanase
EU approval, minimum content Piglets 
endo-1,4-beta-xylanase: 270 U/kg feed 
endo-1,3(4)-beta-glucanase: 70 U/kg feed

endo-1,4-beta-glucanase: 80 U/kg feed 
Poultry for fattening other than turkeys for fattening 
endo-1,4-beta-xylanase: 135 U/kg feed 
endo-1,3(4)-beta-glucanase: 35 U/kg feed
endo-1,4-beta-glucanase: 40 U/kg feed

Turkeys for fattening
endo-1,4-beta-xylanase: 135 U/kg feed 
endo-1,3(4)-beta-glucanase: 35 U/kg feed 
endo-1,4-beta-glucanase: 40 U/kg feed

Poultry for laying 
endo-1,4-beta-xylanase: 216 U/kg feed 
endo-1,3(4)-beta-glucanase: 56 U/kg feed 
endo-1,4-beta-glucanase: 64 U/kg feed
End of period of EU authorisation 23 May 2023
EU code 4a1620i
Derived from microorganism Trichoderma reesei (ATCC 74444)
Product storage stability In the unopened original container the product may be stored for 24 months at room temperature
Storage stability in feed Not available
Heat stability For post pelleting application
Package size 1000 ltr containers
Handling Breathing protection and gloves shall be used during handling
Carrier Not available
Producer DSM

List of references

EFSA (2012). Scientific Opinion on the safety and efficacy of Roxazyme G2 G/L (endo-1,4-beta-xylanase, endo-1,4-beta-glucanase and endo-1,4(3)-beta-glucanase) as a feed additive for poultry and piglets. EFSA Journal 2012;10(11):2930

EU (2013). Commission implementing Regulation (EC) No 403/2013 of 2 May 2013 concerning the authorisation of a preparation of endo-1,4-beta-xylanase, endo-1,3(4)-beta-glucanase and endo-1,4-beta-glucanase produced by Trichoderma reesei (ATCC 74444) as a feed additive for poultry for fattening and for laying and for weaned piglets and amending Regulation (EC) No 1259/2004, (EC) No 1206/2005 and (EC) No 1876/2006 (holder of authorisation DSM Nutritional Products). Official Journal of the European Union. L 121/26

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