The Axtra® Phy liquid product from Danisco Animal Nutrition, DuPont was authorised for use in the EU in 2016. The product is authorised for use in the EU in all porcine and poultry species
Manufacturer´s main claims
Improved P release and bioavailability
Reduced effect of anti-nutrients
Table 1. Technical specifications Axtra® PHY
|EU category||Zoo-technical additive|
|Functional group of additives||Digestibility enhancer|
|Active substance||6-phytase (EC 220.127.116.11)|
|Phytase activity in the product||Liquid form: 15,000 FTU / g
Solid form: 20,000 FTU / g
|EU approval, minimum content/kg feed||250 FTU for:
All poultry species
All porcine species (other than suckling piglets)
|End of period of EU authorisation||Liquid form: 29 June 2026 (
Solid form: 14 June 2027
|Production organism||Trichoderma reesei ATCC SD-6528|
|Donor organism||Buttiauxella spp|
|Product storage stability||Liquid form:
The remaining phytase activity after storing the product for 12 months at 25ºC or 40ºC, respectively, was 78% and 71%, respectively
Three batches of the product were kept for 18 months at either 25°C (60% RH) or 40°C (75% RH). When stored at 25°C the residual phytase activity was 77.5% and at 40°C 28.6%.
|Storage stability in feed||Liquid form:
When sprayed onto feed pellets no reduction in phytase activity could be observed after three months storage at 25ºC or 40ºC, respectively.
Three batches of the product were added to a vitamin/mineral premix and stored for 6 months at 25°C (60% RH). After 6 months of storage time, the drop in phytase activity ranged from no loss at all to 14%.
Three batches of the product were added to mash feed based on maize and soya bean meal. Samples of the feed were kept for three months at 25°C (60% RH). After three months of storage, the residual phytase activity was reduced by 8 to 15%.
|Heat stability||Liquid form:
Only for post-pellet application
Feed based on maize and soya beans supplemented with the phytase product was pelleted at 95°C. The loss of phytase activity due to the heat treatment ranged from 2 to 13%.
Ten subsamples of each feed used for the stability test were collected and analysed. The analytical results showed that the coefficient of variance ranged from 5.8 to 6.7%
Ten subsamples from two different batches of mash were supplemented with the product. The analytical results showed a coefficient of variation of 10%.
Sorbitol, sodium chloride, potassium sorbate, sodium benzoate and water
Fermentation product, sodium sulfate, polyvinyl alcohol, talc and sodium phytate
|Handling||May cause sensitisation by inhalation.|
|Producer||Danisco Animal Nutrition|
List of references
EFSA (2015). Scientific Opinion on the safety and efficacy of Axtra® PHY 15000 L (6-phytase) as a feed additive for poultry and porcine species. EFSA Journal 2015;13(11):4275
EFSA (2016). Safety and efficacy of Axtra® PHY 20000 TPT2 (6-phytase) as a feed additive for poultry and porcine species. EFSA Journal 2016;14(11):4625
EU (2016). Commission Implementing Regulation (EU) 2016/899 of 8 June 2016 concerning the authorisation of a 6-phytase produced by Trichoderma reesei (ATCC SC-6528) as a feed additive for all poultry species and porcine species (other than suckling piglets) holder of authorisation Danisco (UK) Ltd. Official Journal of the European Union L152, 15-17
EU (2017). Commission Implementing Regulation (EU) 2017/896 of 24 May 2017 concerning the authorisation of a preparation of 6-phytase, produced by Trichoderma reesei (ATCC SD-6528) as a feed additive in solid form for all poultry species and all porcine species (other than suckling piglets) (holder of the authorisation Danisco (UK) Ltd). Official Journal of the European Union. L 138/123-125
Menezes-Blackburn, D.; Gabler, S. & Greiner, R. (2015). Performance of seven commercial phytases in an in vitro simulation of poultry digestive tract. Journal of agricultural and food chemistry.