Natuphos E is a product containing 6-phytase. The product is available in various concentrations and three different product forms, powder, granulated and liquid. The granulated form is more suitable for pelleted feed due to higher heat stability.

Manufacturer´s main claims
Makes phytate bound phosphorus available
Releases energy, calcium, copper, zinc, magnesium and amino acids
Improves feed conversion

Table 1. Technical specifications Natuphos® E 

EU category Zoo-technical additive
Functional group of additives Digestibility enhancer
Active substance 6-Phytase (EC 3.1.3.26)
Phytase activity in the product 5,000 or 10,000 FTU/g
EU approval, minimum content No maximum content for any of the animal categories below
Piglets: 125 FTU/kg feed  
Pigs for fattening: 100 FTU/kg feed
Sows: 100 FTU/kg feed
Minor porcine species for fattening or for reproduction: 100 FTU/kg feed
Chickens for fattening: 125 FTU/kg feed
Chickens reared for laying: 125 FTU/kg feed
Turkeys for fattening: 125 FTU/kg feed
Turkeys reared for breeding: 125 FTU/kg feed
All other avian species (excluding laying birds): 125 FTU/kg feed
All avian species for laying: 200 FTU/kg feed
End of period of EU authorisation All species above except all avian species for laying:
28 March 2028
All avian species for laying: 30 July 2030
EU code 4a27
Derived from microorganism Bacterial phytase produced by Aspergillus niger,  (DSM 25770)
Product storage stability Three batches of Natuphos 5000 E (powder form) were stored for 24 months at either 20°C or 30°C in closed paper bags. 
Recovery rates:
6 months at 20°C: >95%
18 months at 20°C: >85%
24 months at 20°C: >80%
3 months at 30°C: >70%
6 months at 30°C: <50%

The granulated form was tested also tested using three batches each of Natuphos 5000 E G and Natuphos 10000 E G. Product samples were stored for up to 15 months in closed plastic bags for up to 15 months at either 20°C or 30°C.
Recovery rates:
6 and 15 months at 20°C: >90% regardless of formulation
2 months at 30°C: >84% (5000 E G)
2 months at 30°C: 85% (10000 E G)
6 months at 30°C: >75% (5000 E G)
6 months at 30°C: 68% (10000 E G)
15 months at 30°C: >49% (5000 E G)
15 months at 30°C: 41% (10000 E G)
Storage stability in feed Three batches of the product as either powder or granulate were added to a mineral premixture and stored for in closed paper bags for 6 months at 20°C or 30°C, respectively. After 6 months the residual activity was >78% for powder and >90% for granulate at 20°C. When stored at 30°C the residual activity for the powder form was very low at 20-23%. The activity in the granulated form was >78%.

Three batches of the product as either powder or granulate were added to a complete feed either as mash or pelleted. These samples were stored for in closed paper bags for 16 weeks at 20°C or 30°C, respectively. 
Recovery rates:
Mash feed 20°C: 93%
Pelleted feed 20°C: 90%
Mash feed 30°C: 82%
Pelleted feed 30°C: 73% 
Heat stability Both the powder form as well as the granulated form were tested at three different temperatures: 80°C, 85°C and 90°C.
Recovery rates:
80°C powder: 85%
80°C granulated: 82% (Natuphos E 5000 G)
80°C granulated: 98% (Natuphos E 10000 G)
90°C powder: 50%
90°C granulated: 70% (Natuphos E 5000 G)
90°C granulated: 88% (Natuphos E 10000G)
Carrier and density Liquid form: glycerol, sodium benzoate, water
Powder form: wheat bran, magnesium sulfate, vegetable oil
Granulated form: starch, polyvinyl alcohol, gum arabic, was-based coating agent, water
Bulk density of the powder form is 348 kg/m3
Bulk density of granulated form is 650 kg/m3
Homogeneity and particle size Five batches of the 5000 E product showed a mean value of 5,372 FTU/g product with a CV of 3.5%.
Five batches of the 5000 E G product showed a mean value of 7,770 FTU/g product with a CV of 16%
Five batches of the 10000 E G product showed a mean value of 12,010 FTU/g product with a CV of 6.4% 

Mean particle size in the powder is 368-374 µm. The content of particles below 100µm, 50µm and 10µm was 5%, 2.6% and 0.1%, respectively.
Mean particle size in the granulated form is 600µm. More than 90% of the particles are smaller than 850µm, <710 µm >80%, <600 µm >50% and below 63 µm: 0.01 - 0.08%
Handling It is recommended to use personal protective equipment including breathing protection
Producer BASF

List of references

EFSA (2017). Safety and efficacy of Natuphos® E (6-phytase) as a feed additive for avian and porcine species . EFSA Journal 2017;15(11):5024

EU (2018). Commission implementing Regulation (EU) 2018/338 of 7 March 2018 concerning the authorisation of a preparation of 6-phytase, produced by Aspergillus niger (DSM 25770) as feed additive for chickens for fattening, chickens reared for laying, pigs for fattening, sows, minor porcine species for fattening or for reproduction, turkeys for fattening, turkeys reared for breeding, all other avian species, (excluding laying birds) and weaned piglets (holder of the authorisation BASF SE). Official Journal of the European Union, L65/17-20

EU (2019). Commission implementing Regulation (EU) 2019/1290 of 31 July 2019 amending Implementing Regulation (EU) 2018/338 as regards the minimum content of a preparation of 6-phytase, produced by Aspergillus niger (DSM 25770) as a feed additive for chickens for fattening or reared for laying (holder of authorisation BASF SE). Official Journal of the European Union. L203/6-7

EU (2020). Commission implementing Regulation (EU) 2020/992 of 9 July 2020 concerning the authorisation of a preparation of 6-phytase, produced by Aspergillus niger (DSM 25770) as feed additive for all avian species for laying (holder of authorisation BASF SE). Official Journal of the European Union. L221/73-75