AFSA Phytafeed® is a product that contains 6-phytase. The product is authorised for use in the EU in chickens and turkeys. AFSA Phytafeed® is available both in dry and liquid forms.

Table 1. Technical specifications APSA Phytafeed® 20 000 (solid or Liquid)

EU category Zoo-technical additives
Functional group of additives Digestibility enhancer
Active substance 6-Phytase (EC 3.1.3.26)
Phytase activity in the product Solid product: 20 000 U/g
Liquid product: 20 000 U/ml
EU approval, minimum content Minimum 250 U/kg feed for all species below:
Chickens for fattening
Chickens reared for laying and breeding
Minor poultry species for fattening or reared for laying or breeding purposes
Turkeys for fattening
Turkeys reared for breeding

Piglets (suckling and weaned: 1000 U/kg feed
Minor porcine species (suckling and weaned): 1000 U/kg feed
End of period of EU authorisation 25 February 2030 for:
Chickens for fattening
Chickens reared for laying and breeding
Minor poultry species for fattening or reared for laying or breeding purposes

22 October 2030 for:
Turkeys for fattening
Turkeys reared for breeding
Piglets (suckling and weaned
Minor porcine species (suckling and weaned)
EU code 4a31
Production organism Genetically modified Komagataella phaffii, CGMCC 12056
Product storage stability Three batches of the solid product were stored for 12 months at either 25°C or 40°C in closed containers. At 25°C 85% of the initial phytase was detected after 12 months. After 6 months of storage at 40°C, only 40% of the initial content was left.
Three batches of the liquid product were also stored for up to 24 months at various temperatures. When stored for 12 months at 5°C, 95% of the initial phytase activity was recovered. Samples stored at 20°C for 6 months lost 15% of the phytase activity. 
Storage stability in feed Three batches of the solid formulation were added to vitamin and mineral premixture. Samples of the premix were stored for 6 months at 25°C. The residual phytase activity was 84%. 
The storage stability of both the solid and liquid forms was studied in complete mash and granulated feed for chickens for fattening.  After 3 months of storage, no loss of phytase activity could be detected in neither the meal feed nor the granulated feed.
Heat stability Complete mash feed for chickens for fattening with the addition of the solid product was granulated at 60-65°C. After granulation, no loss of phytase activity could be detected. 
Homogeneity Five batches of the solid product were analysed and the mean phytase activity was 23,370 U/g with a CV of 5.6%. The mean particle size in three different batches varied from 227 to 243 µm. No particles were under 10 µm and less than 1% were smaller than 50 µm.
Bulk density was 620 kg/m3
Five batches of the liquid product were also analysed to study variation in the phytase content. The mean content was 23,188 U/ml with a CV of 4.3% 
Subsamples of the feed used for the stability studies were used to study homogeneity of the product when mixed into a premixture or final feed. When mixed into a vitamin and mineral premixture a CV of 9.6% could be detected. In the mash feed, a CV of 8% could be obtained with the solid product and 6% with the liquid product.
Package size 25 kg bags
Handling May cause sensitisation by inhalation.  Wear suitable protective equipment.
Producer Andrés Pintaluba S.A.

List of references

EFSA (2019). Safety and efficacy of APSA PHYTAFEED® 20,000 GR/L (6-phytase) as a feed additive for chickens for fattening, chickens reared for laying and minor poultry species. EFSA Journal 2019;17(5):5692

EFSA (2019). Safety and efficacy of APSA PHYTAFEED® 20,000 GR/L (6-phytase) as a feed additive for turkeys for fattening, turkeys reared for breeding and minor poultry species. EFSA Journal 2019;17(11):5893

EU (2020). Commission Implementing Regulation (EU) 2020/150 of 4 February concerning the authorisation of the preparation of 6-phytase produced by a Komagataella phaffii CGMCC 12056 as a feed additive for chickens for fattening, chickens reared for laying and for breeding and minor poultry species for fattening or reared for laying or for breeding purposes (holder of authorisation Andrés Pintaluba S.A.). Official Journal of the European Union, L33/9-11

EU (2020). Commission Implementing Regulation (EU) 2020/1376 of 1 October 2020 concerning the authorisation a preparation of 6-phytase, produced by Komagataella phaffii (CGMCC 12056) as feed additive for turkeys for fattening, turkeys reared for breeding, piglets (suckling and weaned) and minor porcine species (holder of the authorisation Andrés Pintaluba S.A) Official Journal of the European Union. L319/26-28