Introduction
The product Amylofeed contains three different enzymatic activities: beta-glucanase, beta-xylanase and alpha-amylase. The product is authorised for use in the EU in piglets.
Manufacturer´s main claims
Increases digestibility of nutrients
Improves daily weight gain and feed conversion
Reduces stress at weaning and diarrhoea
Technical specifications of APSA Amylofeed
EU category
Zootechnical additives
Functional group of additives
Digestibility enhancers
Active substance
Endo-1,3(4)-beta-glucanase (EC 3.2.1.6)
Endo-1,4-beta-xylanase (EC 3.2.1.8)
Alpha-amylase (EC 3.2.1.1)
Enzyme activity in the product
900 U/g endo-1,3(4)-beta-glucanase
1000 U/g endo-1,4-beta-xylanase
3,000 U/g alpha-amylase
EU approval, minimum content
Piglets (weaned):
Endo-1,3(4)-beta-glucanase: 450 U/kg feed
Endo-1,4-beta-xylanase: 500 U/kg feed
Alpha-amylase: 1,500 U/kg feed
End of period of EU authorisation
8 November 2028
EU code
4a1612i
Production organism
Beta-glucanase: Aspergillus niger (NRRL 25541)
Beta-xylanase: Aspergillus niger (NRRL 25541)
Alpha-amylase: Aspergillus oryzae (ATCC 66222)
Product storage stability
The rate of recovery was studied in three batches of the product at two different temperatures. After two years of storage at 25°C, the activity was 77, 69 and 87 % for glucanase, xylanase and amylase, respectively. After four months of storage at 40°C, the recovery rate was 57, 63 and 67 % for glucanase, xylanase and amylase, respectively.
Storage stability in premix and feed
When the product was added to a vitamin-mineral premix and stored for six months at 25°C, the recovery rate for glucanase, xylanase, and amylase was 66, 73, and 77%, respectively. When stored at 40°C, the residual activity was below 50% for all enzymes. Storage of complete feed supplemented with the product for three months at 25°C reduced the enzymatic activity to 93 % of the initial activity.
Heat stability
Pelleting at 60 - 65°C reduced the activity of glucanase, xylanase and amylase to 49, 34 and 29 %, respectively
Package size
25 kg
Handling
The EU Commission states: "For users of the additive and premixtures, feed business operators shall establish operational procedures and organisational measures to address potential risks resulting from their use. Where those risks cannot be eliminated or reduced to a minimum by such procedures and measures, the additive and premixtures shall be used with personal protective equipment including skin, eyes and breathing protections."
Manufacturer
Andres Pintaluba
List of references
EFSA (2013). Scientific Opinion on the Safety and Efficacy of Amylofeed® (endo-1,3(4)-beta-glucanase), endo-1,4-beta-xylanase and alpha-amylase) as a feed additive for piglets and young minor porcine species. EFSA Journal 2013;11(10):3430
EU (2018). Commission Implementing Regulation (EU) 2018/1566 of 18 October 2018 concerning the authorisation of endo-1,3(4)-beta-glucanase and endo-1,4-beta-xylanase produced by Aspergillus niger (NRRL 25541) and alpha-amylase produced by Aspergillus niger (ATCC 66222) as a feed additive for weaned piglets and minor porcine species (weaned) and amending Regulation (EC) No 1453/2004 (holder of authorisation Andres Pintaluba S.A). Official Journal of the European Union. L 262/27-29