Capsozyme SB Plus

Introduction  
The Capsozyme SB Plus product contains two enzymes, xylanase and galactosidase. Capsozyme SB Plus is aimed for use in diets based on cereals and legumes.

Manufacturer´s main claims
The enzyme solution to improve diets with soya.

Technical specifications of Capsozyme SB Plus

EU category
Zootechnical additives

Functional group of additives
Digestibility enhancers

Active substance
Endo-1,4-beta-xylanase (EC 3.2.1.8)
alpha-galactosidase (EC 3.2.1.22)

Enzymatic activity in the product
Xylanase: 50 AXC/g
Galactosidase: 40 GALU/g

EU approval, minimum content per kg feed
18 AXC
14 GALU

Chickens for fattening
Chickens reared for laying
Minor Poultry species for fattening and reared for laying
Ornamental birds

End of period of EU authorisation
26 September 2032

EU code
4a33

Production organism
Trichoderma longibrachiatum (DSM 33574)
Aspergillus tubingensis (ATCC SD 6740)

Product storage stability
Samples from three product batches were kept in plastic bags at room temperature or 40°C for 12 months. At room temperature, 7% of the xylanase and 9% of the galactosidase activity was lost. When stored at 40°C, the enzyme activity loss increased to 12% for the xylanase and 14% for the galactosidase.

Storage stability in premix and feed
The product was added to a vitamin/mineral premix and stored for six months at 25°C. After six months, 10% of the xylanase and 3% of the galactosidase were lost.
The stability of the product in chicken feed was observed for an unspecified duration. 3% less xylanase and 12% less galactosidase were observed in the mash feed. In pelleted feed, 12% less xylanase and 8% less galactosidase were seen.

Heat stability
Mash feed based on maize and soybean meals was pelleted at 70°C. Eight per cent of the xylanase activity was lost during pelleting. For the galactosidase, the activity was reduced by 11%.

Physical properties
The product contains 2% xylanase, 2% galactosidase, 95% kieselgur and 1% dextrin. The variation of enzyme activity was tested in samples from six batches. The average content of GALU was 45 GALU/gram, with a CV of 6%. The average content of galactosidase was 58 AXC/g, with a CV of 8%.
Laser diffraction was used to measure the particle size distribution. Forty-two per cent of the particles were <100 µm, 10 % < 10 µm.
The dusting potential was measured to <0.1 g/m3. The bulk density ranged from 575 to 600 kg/m3.

Homogeneity
The product was added to a pig diet to test the capacity to mix homogeneously. Ten subsamples of the feed were collected and analysed. The CV was 6% for the xylanase and 8% for the galactosidase.

Handling
The EU authorisation states: "For users of the additive and premixtures, feed business operators shall establish operational procedures and organisational measures to address potential risks resulting from their use. Where those risks cannot be eliminated or reduced to a minimum by such procedures and measures, the additive and premixtures shall be used with personal protective equipment, including skin, eyes and breathing protection."

Manufacturer
ITPSA

List of references

EFSA (2020). Safety and efficacy of Capsozyme SB Plus (a-galactosidase and endo-1,4-b-xylanase) as a feed additive for poultry species for fattening or reared for laying and ornamental birds. EFSA Journal 2020;18(4):6086

EU (2022). COMMISSION IMPLEMENTING REGULATION (EU) 2022/1470 of 5 September 2022 concerning the authorisation of endo-1,4-beta-xylanase produced by Trichoderma longibrachiatum CBS 139997 and alpha-galactosidase produced by Aspergillus tubingensis ATCC SD 6740 as a feed additive for chickens for fattening, chickens reared for laying, minor poultry species for fattening and reared for laying, and ornamental birds (holder of the authorisation Industrial Técnica Pecuaria S.A.). Official Journal of the European Union, L 231/109-112