Introduction
Enzy Carboplus® is an NSP-degrading product from the company Kaesler Nutrition. The xylanase and glucanase contained in the product are suitable for diets rich in arabinoxylans and beta-glucans.
Manufacturer´s main claims
Lower feed costs while maintaining animal performance
Improves feed conversion
Heat stable up to 90°C
Technical specifications of Enzy Carboplus® (solid P and Liquid L)
EU category
Zootechnical additives
Functional group of additives
Digestibility enhancers
Active substance
Endo-1,4-beta-xylanase (EC 3.2.1.8)
Endo-1,3(4)-beta-glucanase (EC 3.2.1.6)
Enzymatic activity in the product
25 000 LXU/g
2 200 LGU/g
EU approval, minimum content
Chickens for fattening: 4250 LXU/kg and 375 LGU/kg feed
Chickens reared for laying: 4250 LXU/kg and 375 LGU/kg feed
Turkeys for fattening: 1400 LXU/kg and 120 LGU/kg feed
All avian species reared for laying or for breeding other than chickens reared for laying: 1400 LXU/kg and 120 LGU/kg feed
Weaned piglets: 700 LXU/kg and 60 LGU/kg feed
Minor porcine species (weaned): 700 LXU/kg and 60 LGU/kg feed
End of period of EU authorisation
21 August 2028 for the below species:
Chickens for fattening
Chickens reared for laying
Turkeys for fattening
All avian species reared for laying or for breeding other than chickens reared for laying
Weaned piglets
Minor porcine species (weaned)
EU code
4a28
Production organism
Xylanase: Komagataella pastoris (CBS 25376)
Glucanase: Komagataella pastoris (CBS 26469)
Product storage stability
Three batches of the solid product were stored in closed containers for 24 months at 25°C or for six months at 40°C. At both temperatures, the rate of recovery was >80%. The stability of the liquid product was also tested using three different batches. Samples of the three batches were stored at either 5°C for 24 months or at 25°C for six months. At either temperature, >90% of the xylanase activity was recovered. At 25°C and six months, 80% of the glucanase could be recovered, and 65% at 5°C and 24 months.
Storage stability in premix and feed
Three batches of the product were supplemented to vitamin/mineral premixes and stored in paper bags with inside polyethylene coating for six months at 25°C. After six months,>84% of the initial xylanase activity was recovered, whereas 87% of the glucanase activity could be recovered.
The stability of the additive was also studied when the solid or the liquid product was added to the feed. The solid product was added to a mash feed and pelleted, and the liquid product was added to a pelleted feed after pelleting. Samples of the feed supplemented with solid or liquid products were collected and stored for three months at 25°C. More than 95% of the initial enzymatic activity could be recovered.
Heat stability
The stability of the solid product was tested using three batches of the product in a mash feed. The mash feed was pelleted at an unknown temperature. After pelleting, 90% of the initial xylanase activity was left, whereas 70% of the glucanase could be recovered.
Homogeneity
The capacity of the solid product to be evenly distributed in the feed was tested using the ten subsamples from the stability tests. The analyses showed that the CV was <8 %.
Physical properties
Particle size distribution was studied using laser diffraction. Eighty per cent of the particles were <100 μm, 5 % <10 μm. The dusting potential was measured at 5.07 g/m3
Carrier
Solid formulation: 88.5% corn starch; 1.5% enzyme; 10% moisture
Liquid formulation: 15% glycerol; 5% sodium chloride; 0.3% sodium benzoate; 0.15% potassium benzoate; 1.5% enzyme; water constitutes the remaining part.
Handling
The European Commission states: "For users of the additive and premixtures, feed business operators shall establish operational procedures and organisational measures to address potential risks from their use. Where those risks cannot be eliminated or reduced to a minimum by such procedures and measures, the additive and premixtures shall be used with personal protective equipment."
Manufacturer
Kaesler Nutrition
List of references
EFSA (2017). Safety and efficacy of ENZY CARBOPLUS® (endo-1,4-beta-xylanase) and endo-1,3(4)-beta-glucanase) as a feed additive for avian species, weaned piglets and minor weaned porcine species. EFSA Journal 2017;15(12):5097
EU (2018). Commission Implementing Regulation (EU) 2018/1090 of 31 July 2018 concerning the authorisation of a preparation of endo-1,4-beta-xylanase and endo-1,3(4)-beta-glucanase produced by Komagataella pastoris (CBS 25376) and Komagataella pastoris (CBS 26469) as a feed additive for chickens for fattening, chickens reared for laying, turkeys for fattening, all avian species reared for laying or for breeding <purposes, weaned piglets and minor porcine species (weaned) (holder of authorisation Kaesler Nutrition GmbH). Official Journal of the European Union. L 195/23-26