Huvezym neXo

Introduction

Huvezym® neXo is an enzymatic complex designed to break down complex fiber structures in diets that include fiber-rich raw materials, alternative feedstuffs, or conventional raw materials requiring additional fibrolytic enzymes for efficient hydrolysis.

As a nutritional tool, Huvezym® neXo enhances nutrient digestibility, which can lead to cost-effective feed reformulation and improved zootechnical performance. Additionally, it supports a healthier gut environment.

Key characteristics include:

  • Increased nutrient digestibility in diets high in complex fibers.
  • Potential for feed cost savings through diet reformulation.
  • Improved zootechnical performance and support for gut health.

Manufacturer´s main claims 

Huvezym® neXo is an advanced enzymatic complex designed to break down complex fiber structures in various diets, including those with fiber-rich or alternative feedstuffs. This enzyme complex enhances nutrient digestibility, allowing for cost-effective feed reformulation and improved animal performance. Key benefits include increased nutrient digestibility, significant feed cost savings, and improved zootechnical performance while supporting gut health.

Technical specifications of Huvezym® neXo (G and L)

EU category
Zootechnical additives

Functional group of additives
Digestibility enhancers

Active substance
Endo-1,4-beta-xylanase (EC 3.2.1.8)
Endo-1,4-beta-glucanase (EC 3.2.1.4)
Xyloglucan-specific endo-beta-1,4-glucanase (EC 3.2.1. 151)

Enzymatic activity in the product
Endo-1,4-beta-xylanase 15,000 EPU/g
Endo-1,4-beta-glucanase 1,000 CU/g
Xyloglucan-specific endo-beta-1,4-glucanase 1,000 XGU/g

EU approval, minimum content
Poultry for fattening
Poultry reared for laying and reared for breeding
Ornamental birds
Sows of all Suidae species

Endo-1,4-beta-xylanase: 1,500 EPU/kg feed
Endo-1,4-beta-glucanase: 100 CU/kg feed
Xyloglucan-specific endo-beta-1,4-glucanase 100 XGU/kg feed

End of period of EU authorisation
Poultry for fattening
Poultry reared for laying and reared for breeding
Ornamental birds

6 July 2033

Sows of all Suidae species

23 September 2034

EU code

4a39

Production organism

Trichoderma citrinoviride (DSM 33578)

Product storage stability (neXo 100 G)
The shelf life of Huvezym® neXo 100 G was tested using three batches stored in paper bags with a polyethylene lining. The tests were conducted at two different temperatures: 25°C for up to 24 months and 40°C for up to 6 months. Here's what the study found:

  • At 25°C, after 24 months, the enzyme activities decreased slightly:

    • Xylanase activity decreased by an average of 9.3%.
    • Glucanase activity decreased by an average of 9.6%.
    • Xyloglucanase activity decreased by an average of 9.5%.

  • At 40°C, after 6 months, the enzyme activities showed a more noticeable decrease:

    • Xylanase activity decreased by an average of 19.4%.
    • Glucanase activity decreased by an average of 26.2%.
    • Xyloglucanase activity decreased by an average of 25.4%.

Storage (and heat) stability in premix and feed

  1. Vitamin-Mineral Premixture for Poultry and Pigs:

    • Storage Conditions: The additive was stored in polyethylene bags within double paper bags at 25°C for 6 months and at 40°C for 3 weeks.
    • Results at 25°C: After 6 months, the enzyme activity loss was approximately 12-16% for poultry and 13-14% for pigs.
    • Results at 40°C: After 3 weeks, the enzyme activity loss ranged from 16.5-20.6% for poultry and 16.8-18.6% for pigs.

  2. Complete Mash Feed for Poultry:

    • Storage Conditions: The feed was stored similarly at 25°C for 3 months and at 40°C for 3 weeks.
    • Results at 25°C: After 3 months, the enzyme activity loss was around 5-6%.
    • Results at 40°C: After 3 weeks, the enzyme activity loss was approximately 10-11%.
    • Pelleting Effect: Pelleting at 85°C resulted in an enzyme activity loss of about 3-5%.
    • Post-Pelleting Storage at 25°C: After 3 months, the additional enzyme activity loss was around 6-7%.
    • Post-Pelleting Storage at 40°C: After 3 weeks, the additional enzyme activity loss was approximately 11-12%.

  3. Complete Mash Feed for Pigs:

    • Storage Conditions: Similar storage conditions were applied.
    • Results at 25°C: After 3 months, the enzyme activity loss was about 5-6%.
    • Results at 40°C: After 3 weeks, the enzyme activity loss was around 9-11%.
    • Pelleting Effect: Pelleting at 85°C led to an enzyme activity loss of approximately 3-6%.
    • Post-Pelleting Storage at 25°C: After 3 months, the additional enzyme activity loss was around 6-8%.
    • Post-Pelleting Storage at 40°C: After 3 weeks, the additional enzyme activity loss was about 11-13%.

Product storage stability (neXo 100 L)

  1. Shelf Life in Plastic Bottles:

    • Storage Conditions: The product was stored in small plastic bottles at 25°C and 40°C for up to 12 months and 6 months, respectively.
    • Results at 25°C: After 12 months, the enzyme activity loss was approximately 10% for xylanase, 11% for glucanase, and 10.1% for xyloglucanase.
    • Results at 40°C: After 6 months, the enzyme activity loss increased to about 21.4% for xylanase, 26.8% for glucanase, and 26.1% for xyloglucanase.

  2. Stability on Pelleted Feed for Poultry:

    • Storage Conditions: The additive was sprayed onto pelleted feed and stored at 25°C for 3 months and at 40°C for 3 weeks.
    • Results at 25°C: After 3 months, the enzyme activity loss was around 7.7% for xylanase, 8.1% for glucanase, and 8.4% for xyloglucanase.
    • Results at 40°C: After 3 weeks, the enzyme activity loss was approximately 13.8% for xylanase, 13.5% for glucanase, and 14.3% for xyloglucanase.

  3. Stability on Pelleted Feed for Pigs:

    • Storage Conditions: Similar storage conditions were applied as with poultry feed.
    • Results at 25°C: After 3 months, the enzyme activity loss was about 7.7% for xylanase, 7.6% for glucanase, and 8.4% for xyloglucanase.
    • Results at 40°C: After 3 weeks, the enzyme activity loss was around 13.2% for xylanase, 13.8% for glucanase, and 14% for xyloglucanase.

Homogeneity

A study was conducted to assess how evenly Huvezym® neXo 100 G distributes in mash feed for both poultry and pigs. The researchers analyzed 10 subsamples from three batches for each type of feed. Here's what they found:

  • For Poultry:

    • The coefficient of variation for xylanase ranged between 10.4% and 12%.
    • For glucanase, it ranged between 9.6% and 10%.
    • For xyloglucanase, it ranged between 8.9% and 10.7%.

  • For Pigs:

    • The coefficient of variation for xylanase ranged between 9% and 10.3%.
    • For glucanase, it ranged between 9.2% and 10.1%.
    • For xyloglucanase, it ranged between 9% and 11.4%.

These coefficients of variation indicate the degree of homogeneity in the distribution of the enzymes within the mash feed for both poultry and pigs. Lower percentages suggest a more uniform distribution.

Physical properties

  1. Huvezym® neXo 100 G (Granules):

    • Appearance: Beige to brownish granules.
    • Density: Poured density ranges from 420 to 470 kg/m³, and tapped density ranges from 490 to 540 kg/m³.
    • Dusting Potential: On average, 68.3 mg/m³, with a range of 55 to 80 mg/m³.
    • Particle Size Distribution: Approximately 88.9% of particles are between 100 μm and 800 μm in size, while 11.1% of particles are below 100 μm in diameter.

  2. Huvezym® neXo 100 L (Liquid):

    • Appearance: Yellowish to brown liquid.
    • Density: Ranges from 1,100 to 1,300 kg/m³, with analysis in six batches showing values between 1,150 and 1,200 kg/m³.
    • Viscosity: Average viscosity is 12.1 cP, with a range of 12.0 to 12.2 cP.

Handling
The European Commission states: "For users of the additive and premixtures, feed business operators shall establish operational procedures and organisational measures to address the potential risks resulting from their use. Where those risks cannot be eliminated by such procedures and measures, the additive and premixtures shall be used with personal breathing, skin and eye protective equipment."

Manufacturer
Huvepharma

List of references

EFSA (2022). Safety and efficacy of a feed additive consisting of endo-1,4-beta xylanase, endo-1,4-beta-glucanase and xyloglucan-specific-endo-beta-1,4-glucanase produced by Trichoderma citrinoviride DSM 33578 (Huvezym® neXo 100 G/L) for all poultry species, ornamental birds and piglets (weaned and suckling) (Huvepharma EOOD). EFSA Journal 2022;20(12):7702

EU (2023). COMMISSION IMPLEMENTING REGULATION (EU) 2023/1169 of 15 June 2023 concerning the authorisation of a preparation of endo-1,4-beta-xylanase, endo-1,4-beta-glucanase and xyloglucan-specific endo-beta-1,4-glucanase produced by Trichoderma citrinoviride DSM 33578 as a feed additive for poultry for fattening, poultry reared for laying and reared for breeding, and ornamental birds (holder of authorisation: Huvepharma EOOD). Official Journal of the European Union, L155/11-14

EU (2024). REGULATION (EU) 2024/2180 of 2 September 2024 concerning the authorisation of a preparation of endo-1,4-beta-xylanase, endo-1,4-beta-glucanase and xyloglucan-specific-endo-beta-1,4-glucanase produced with Trichoderma citrinoviride DSM 33578 as a feed additive for sows of all Suidae species (holder of authorisation: Huvepharma EOOD). Official Journal of the European Union, http://data.europa.eu/eli/reg_impl/2024/2180/oj