Introduction
Natugrain contains two different enzymatic activities: xylanase and beta-glucanase. The product is authorised for use in the EU in piglets, pigs for fattening and in all major avian species.
Natugrain is available both in dry (Natugrain TS) and liquid forms (Natugrain TS L).
Manufacturer´s main claims
Improves the nutritional value of diets rich in NSP, e.g. wheat, barley, rye and triticale
Technical specifications of Natugrain TS Powder and Liquid
EU category
Zootechnical additives
Functional group of additives
Digestibility enhancers
Active substance
endo-1,4-β-xylanase (EC 3.2.1.8)
endo-1,4-β-glucanase (EC 3.2.1.4)
Enzymatic activity in the product
Xylanase: 5,600 TXU/g
Glucanase: 2,500 TGU/g
EU approval, minimum content
560 TXU and 250 TGU/kg feed
Lactating sows
Piglets and pigs for fattening
Turkeys: 560 TXU/kg and 250 TGU/kg feed
280 TXU/ and 125 TGU/kg feed
Chickens for fattening:
Laying hens
All minor poultry species for fattening and laying
Ornamental birds
Chickens reared for laying
All minor avian species for laying
End of period of EU authorisation
Pigs for fattening: 12 January 2024*
Lactating sows: 22 October 2030
8 July 2031
Piglets
Chickens for fattening
Laying hens
All minor poultry species for fattening and laying
Ornamental birds
Chickens reared for laying
Turkeys
*: Following the provisions of Art. 14 of Reg. (EC) No 1831/2003, an application, in accordance with Article 7, has been submitted
EU code
4a7
Production organism
Xylanase: Aspergillus niger (CBS 109.713)
Glucanase: Aspergillus niger (DSM 18404)
Product storage stability
Three batches of Natugrain TS were stored for 12 months at either 20°C or 35°C. Negligible amounts of activity were lost at 20°C, whereas 20% of the xylanase activity was lost when stored at 35°C for six months.
No losses were observed in the liquid application when stored for 12 months at either 20°C or 35°C.
Storage stability in premix and feed
No losses were observed when added to mash feed and stored for 16 months at 20°C or six months at 35°C. When added to pelleted feed, the activity was similar to mash when stored for 16 months at 20°C. If stored at 35°C, no activity was lost after ten months.
The liquid application was added to pelleted feed and stored for 16 weeks at either 20°C or 35°C. At 20°C, there was no loss, whereas at 35°C, xylanase activity decreased by 1 % and glucanase activity by 12%.
Heat stability
Pelleting at 80-85°C reduced the xylanase activity in the solid product by 12%.
The liquid product is intended for post-pelleting application
Homogeneity
Only the dry formulation was tested for its homogeneous mixing ability in the feed. The coefficient of variance was less than 9% in mash and pelleted feed.
Physical properties
The dry product's particle size distribution was analysed. The test showed that less than 10% of the particles were smaller than 150 µm, and there were no particles smaller than 75 µm.
Carrier
Solid formulation: Wheat middlings and vegetable oil
Liquid formulation: Sorbitol, glycerol, sodium-benzoate and water
Package size
20 kg bags or 350 kg big plastic bags
125 kg plastic drum or 1000 kg bulk container
Handling
The European Commission states: "For safety: breathing protection, glasses and gloves shall be used during handling."
Manufacturer
BASF
List of references
EFSA (2009). Safety and efficacy of Natugrain® TS (endo-1,4-β-xylanase and endo-1,4-β-glucanase) as a feed additive for piglets (weaned), chickens for fattening, laying hens, turkeys for fattening and ducks. The EFSA Journal (2008) 914, 1-19
EU (2009). Commission Regulation (EC) No 271/2009 of 2 April 2009 concerning the authorisation of a preparation of endo-1,4-beta-xylanase and endo-1,4-beta-glucanase as a feed additive for weaned piglets, chickens for fattening, laying hens, turkeys for fattening and ducks for fattening (holder of authorisation BASF SE). Official Journal of the European Union L91/5
EU (2011). Commission Implementing Regulation (EU) No 1068/2011 of 21 October 2011 concerning the authorisation of an enzyme preparation of endo-1,4-beta-xylanase produced by Aspergillus niger (CBS 109.713) and endo-1,4-beta-glucanase produced by Aspergillus niger (DSM 18404) as a feed additive for chickens reared for laying, turkeys for breeding purposes, turkeys reared for breeding, other avian species (other than ducks for fattening) and ornamental birds (holder of authorisation BASF SE). Official Journal of the European Union. L 277/11-13
EU (2013). Commission Implementing Regulation (EU) No 1404/2013 of 20 December 2013 concerning the authorisation of a preparation of endo-1,4-beta-xylanase produced by Aspergillus niger (CBS 109.713) and endo-1,4-beta-glucanase produced by Aspergillus niger (DSM 18404) as a feed additive for pigs for fattening. (holder of authorisation BASF SE). Official Journal of the European Union. L349/88-90
EU (2014). Commission Implementing Regulation (EU) No 1070/2014 of 10 October 2014 amending Regulation (EC) No 271/2009 as regards the minimum content of the preparation of endo-1-4-beta-xylanase produced by Aspergillus niger (CBS 109.713) and endo-1,4-beta-glucanase produced by Aspergillus niger (DSM 18404) as a feed additive for laying hens (holder of authorisation BASF SE). Official Journal of the European Union. L 295/47-49
EU (2017). Commission Implementing Regulation (EU) 2017/950 of 2 June 2017 amending Implementing Regulation (EU) No 1068/2011 as regards the minimum content of the preparation of endo-1,4-beta-xylanase produced by Aspergillus niger (CBS 109.713) and endo-1,4-beta-glucanase produced by Aspergillus niger (DSM 18404) as a feed additive for chickens reared for laying and all avian species for laying (holder of authorisation BASF SE). Official Journal of the European Union. L 143/5-8
EU (2020). Commission Implementing Regulation (EU) 2020/1371 of 1 October 2020 concerning the authorisation of a preparation of endo-1,4-beta-xylanase and endo-1,4-beta-glucanase as a feed additive for lactating sows (holder of the authorisation BASF SE). Official Journal of the European Union. L 319/8-10
EU (2021). Commission Implementing Regulation (EU) 2021/981 of 17 June 2021 concerning the renewal of the authorisation of a preparation of endo-1,4-beta-xylanase produced by Aspergillus niger CBS 109.713 and endo-1,4-beta-glucanase produced by Aspergillus niger DSM 18404 as a feed additive for poultry species, ornamental birds and weaned piglets (holder of authorisation: BASF SE), and repealing Regulation (EC) No 271/2009 and Implementing Regulation (EU) No 1068/2011. Official Journal of the European Union L 216/135-137