Introduction
Ronozyme® MultiGrain contains two different beta-glucanases and one xylanase. The product is authorised for use in the EU in piglets and various avian species.
Ronozyme® MultiGrain is available in various concentrations and in dry and liquid forms:
Manufacturer´s main claims
Improves the utilization of energy in cereals and cereal by-products
Improves piglet performance
Technical specifications of Ronozyme® MultiGrain
EU category
Zootechnical additives
Functional group of additives
Digestibility enhancers
Active substance
endo-1,4-beta-xylanase (EC 3.2.1.8)
endo-1,3(4)-beta-glucanase (EC 3.2.1.6)
endo-1,4-beta-glucanase (EC 3.2.1.4)
Enzymatic activity in the product
2,700 U/g endo-1,4-beta-xylanase
700 U/g endo-1,3(4)-beta-glucanase
800 U/g endo-1,4-beta-glucanase
EU approval, minimum content
Piglets and turkeys for fattening
endo-1,4-beta-xylanase: 270 U/kg feed
endo-1,3(4)-beta-glucanase: 70 U/kg feed
endo-1,4-beta-glucanase: 80 U/kg feed
All poultry for fattening other than turkeys for fattening
endo-1,4-beta-xylanase: 135 U/kg feed
endo-1,3(4)-beta-glucanase: 35 U/kg feed
endo-1,4-beta-glucanase: 40 U/kg feed
All poultry species for laying
endo-1,4-beta-xylanase: 216 U/kg feed
endo-1,3(4)-beta-glucanase: 56 U/kg feed
endo-1,4-beta-glucanase: 64 U/kg feed
End of period of EU authorisation
4 February 2034
EU code
4a1602i
Production organism
Trichoderma reesei (ATCC 74444)
Product storage stability
In the unopened original container, the product may be stored for 36 months at a temperature below 15°C.
Storage stability in premix and feed
According to the manufacturer, Ronozyme® MultiGrain retains 98% of the initial enzymatic activity when mixed into a vitamin premix. The manufacturer claims that Ronozyme® MultiGrain retains 84% of the initial activity after three months when mixed into piglet mash feed.
Heat stability
The heat stability of the dry product formulation was tested at 80°C and 90°C. After pelleting, the enzyme activity was above 82%
Physical properties
A dusting potential test showed that Ronozyme MultiGrain's average dusting potential is 3.3 mg/m3, ranging from 0 to 10 mg/m3.
Laser diffraction was used to determine the particle size distribution. The test showed that the average particle size was 539 µm with no particles smaller than 250 µm.
Package size
20 kg paper sacks
Handling
The European Commission states: "For users of the additive and premixtures, feed business operators shall establish operational procedures and organisational measures to address potential risks resulting from their use. Where those risks cannot be eliminated by such procedures and measures, the additive and premixtures shall be used with personal breathing, eye and skin protective equipment."
List of references
EFSA (2012). Scientific Opinion on the safety and efficacy of Roxazyme G2 G/L (endo-1,4-beta-xylanase, endo-1,4-beta-glucanase and endo-1,4(3)-beta-glucanase) as a feed additive for poultry and piglets. EFSA Journal 2012;10(11):2930
EFSA (2023). Safety and efficacy of a feed additive consisting of endo-1,4-beta-xylanase, endo-1,3(4)-beta-glucanase and endo-1,4-beta-glucanase produced by Trichoderma reesei ATCC 74444 (Ronozyme® Multigrain) for use in poultry for fattening, poultry for laying and piglets (weaned) (DSM Nutritional Products). EFSA Journal 2023;21(6)8043
EU (2013). Commission Implementing Regulation (EC) No 403/2013 of 2 May 2013 concerning the authorisation of a preparation of endo-1,4-beta-xylanase, endo-1,3(4)-beta-glucanase and endo-1,4-beta-glucanase produced by Trichoderma reesei (ATCC 74444) as a feed additive for poultry for fattening and for laying and for weaned piglets and amending Regulation (EC) No 1259/2004, (EC) No 1206/2005 and (EC) No 1876/2006 (holder of authorisation DSM Nutritional Products). Official Journal of the European Union. L 121/26
EU (2024). COMMISSION IMPLEMENTING REGULATION (EU) 2024/221 of 12 January 2024 concerning the renewal of the authorisation of a preparation of endo-1,4-beta-xylanase, endo-1,3(4)-
beta-glucanase and endo-1,4-beta-glucanase, produced by Trichoderma reesei ATCC 74444, as a feed additive for all poultry species for fattening, all poultry species for laying and weaned piglets (holder of authorisation: DSM Nutritional Products) and amending Implementing Regulation (EU) No 403/2013. Official Journal of the European Union. http://data.europa.eu/eli/reg_impl/2024/221/oj