Introduction
Ronozyme® ProAct contains a serine protease. The product is available in a coated form (CT) or as a liquid (L).
Manufacturer´s main claims
Increases protein digestibility
Improves protein utilization by the animal
Allow lower-quality protein to be included in the diet
Technical specifications of Ronozyme® ProAct CT and L
EU category
Zootechnical additives
Functional group of additives
Digestibility enhancers
Active substance
Serine protease (EC 3.4.21.-)
Enzymatic activity in the product
Protease activity in the product: 75,000 PROT/g
EU approval, minimum content
End of period of EU authorisation
Chickens for fattening: 21 September 2031
EU code
4a13
Production organism
Bacillus licheniformis (DSM 19670)
Product storage stability
The shelf life of the CT product was measured in samples of the product stored for 18 months in closed vials at temperatures ranging from -18°C to 50°C. The residual activity ranged from >93 to 98%.
The liquid product was stored for 12 months at the same temperature as the CT product. At the end of the storage period, 97, 96, 86, 81 and 42% of the initial activity could be recovered at 10, 25, 35, 40 and 50°C, respectively.
Storage stability in premix and feed
After three months of storage at 25°C of premixes supplemented with the product, 92-93% of the protease activity could be recovered. Mash feed added with the protease was also stored for three months at 25°C. The protease activity after three months was 95%.
Samples of feed supplemented with the liquid product were also stored for three months at 25°C. After three months, 81% of the activity was recovered.
Heat stability
Heat stability was tested at 70°C, 80°C and 90°C. The protease activity was analysed before pelleting and up to three months after pelleting. After three months, 96, 95 and 92% of the initial activity could be recovered when the pelleting temperature was 70°C, 80°C or 90°C, respectively.
Homogeneity
The coefficient of variance was 5.2% in samples of a premix supplemented with the CT product. When the CT product was added to a broiler CV was 5.5%. Adding the L product to a broiler feed revealed a CV of 6.3%.
Carrier
Solid formulation: Sodium sulphate, cellulose, dextrin, sucrose, palm oil
Liquid formulation: Sorbitol, glycerol, potassium sorbate, sodium benzoate
Handling
The European Commission states: "For users of the additive and premixtures, feed business operators shall establish operational procedures and organisational measures to address potential risks by inhalation, dermal contact or eyes contact. Where those risks cannot be eliminated or reduced to a minimum by such procedures and measures, the additive and premixtures shall be used with personal protective equipment, including eyes, skin and breathing protection."
List of references
EFSA (2009). Safety and efficacy of Ronozyme® ProAct (serine protease) for use as a feed additive for chickens for fattening. The EFSA Journal (2009) 1185, 1-15
EFSA (2021). Safety and efficacy of a feed additive consisting of serine protease produced by Bacillus licheniformis DSM 19670 (Ronozyme ® ProAct) for chickens for fattening (DSM Nutritional Products Ltd.). EFSA Journal 2021;19(3):6448
EU (2010). Commission Regulation (EU) No 8/2010 of 23 December 2009 concerning the authorisation of the serine protease produced by Bacillus licheniformis (DSM 19670) as a feed additive for chickens for fattening (Holder of the authorisation DSM Nutritional Products Ltd, represented by DSM Nutritional Products Sp. Z.o.o). Official Journal of the European Union. L3/7-9
EU (2021). Commission Implementing Regulation (EU) 2021/1426 of 31 August 2021 concerning the authorisation of serine protease produced by Bacillus licheniformis DSM 19670 as a feed additive for chickens for fattening (holder of the authorisation DSM Nutritional Products Ltd., represented in the Union by DSM Nutritional Products Sp. z.o.o.). Official Journal of the European Union. L 307/17-19