Introduction
Rovabio® Advance is an enzymatic feed additive designed to enhance the digestibility of animal feed. It features a unique enzymatic profile, including multi-carbohydrase and debranching enzymes, which have been tested under various conditions. The product aims to improve animal performance and profitability. It is suitable for use in the feed of poultry, pigs, and aquaculture species.
Manufacturer´s main claims
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Feed Digestibility: The product is said to enhance the availability of nutrients in feed, particularly improving the utilisation of net energy in corn and wheat-based diets. This leads to better digestive absorption of key nutrients such as starch, protein, and fat.
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Performance and Profitability: It is claimed to improve the feed conversion ratio and body weight gain in animals. Additionally, it is suggested to decrease morbidity and mortality rates.
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Feed Formulation Flexibility: The product allows for the use of a wider range of raw materials in feed formulations at higher inclusion levels for both poultry and swine.
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Gut Health: It is reported to positively influence gut integrity and the microbiome by promoting beneficial bacteria growth through the production of certain oligosaccharides with prebiotic properties.
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Environmental Sustainability: The product claims to reduce the quantity of raw materials used in feed, leading to a lower carbon footprint and better land use. It also suggests a reduction in nutrient excretions, such as nitrogen and phosphorus, into manure.
Technical specifications of Rovabio Advance
EU category
Zootechnical additives
Functional group of additives
Digestibility enhancers
Active substance
endo-1,3(4)-beta-glucanase (EC 3.2.1.6)
endo-1,4-beta-xylanase (EC 3.2.1.8)
Enzymatic activity in the product
Solid form:
15,200 VU/g endo-1,3(4)-beta-glucanase
22,000 VU/g endo-1,4-xylanase
Liquid form:
3,800 VU/ml endo-1,3(4)-beta-glucanase
5,500 VU/ml endo-1,4-xylanase
EU approval, minimum content
Chickens for fattening, chickens reared for laying, minor poultry species for fattening and reared for laying, laying hens, turkeys for fattening and turkeys reared for breeding:
1,100 VU/kg feed endo-1,4-beta-xylanase
760 VU/kg feed endo-1,3(4)-beta-glucanase
End of period of EU authorisation
Chickens for fattening: 19 May 2025*
Chickens reared for laying: 19 May 2025*
Minor poultry species for fattening and reared for laying: 19 May 2025*
Turkeys for fattening: 31 December 2025
Turkeys reared for breeding: 31 December 2025
Laying hens: 28 February 2027
* An application for renewal in accordance with Art. 14 of Regulation (EC) 1831/2003 has been submitted
EU code
4a22
Production organism
Talaromyces versatilis sp. nov. IMI CC 378536
Talaromyces versatilis sp. nov. DSM 26702
Product storage stability
The shelf-life of two formulations was studied over a period of 12 months.
- Sample Details: Samples of 30 grams or milliliters from three batches were used.
- Storage Conditions:
- Packaging: Solid formulations in zip bags and liquid formulations in bottles.
- Environment: Stored at temperatures of 5°C or 20°C with 60% relative humidity.
- Measurements: Enzyme activities were measured in viscosity and DNS units.
- Results:
- No loss in enzyme activity for samples stored at 5°C for 12 months.
- At 20°C, enzyme activity loss was ≤ 8% for the solid formulation and ≤ 11% for the liquid formulation after 12 months.
Storage stability in premix and feed
The stability of ROVABIO® ADVANCE POWDER was evaluated when it was added to two different complete premixes for pigs, both containing choline chloride. The study involved storing samples under the following conditions:
- Storage Duration: 6 months
- Storage Temperatures: 20°C or 30°C
Results for the first premix tested:
- At 20°C:
- Mean loss of 24% for xylanase activity
- Mean loss of 13% for glucanase activity
- At 30°C:
- Mean loss of 36% for xylanase activity
- Mean loss of 25% for glucanase activity
Results for the second premix tested:
- At 20°C:
- No loss of enzyme activity
- At 30°C:
- Mean loss of 15% for xylanase activity
- Mean loss of 10% for glucanase activity
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The stability of ROVABIO® ADVANCE POWDER was assessed when it was added to mash feed for pigs, introduced via a premix containing choline chloride. The study involved storing samples under the following conditions:
- Storage Duration: 4 months
- Storage Temperatures: 20°C and 30°C
- Packaging: Closed packages
Results after 4 months:
- At 20°C:
- Loss in xylanase activity: 7%
- No loss in glucanase activity
- At 30°C:
- Loss in xylanase activity: 4%
- No loss in glucanase activity
Heat stability
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Effect of Heat Treatment at 70°C:
- Loss in xylanase activity: 16%
- No loss in glucanase activity
The stability of ROVABIO® ADVANCE LIQUID was evaluated when it was sprayed onto pelleted feed for fattening poultry at the recommended use level. The study involved storing samples under the following conditions:
- Storage Duration: 4 months
- Storage Temperatures: 20°C and 30°C
- Packaging: Closed packages
Results after 4 months:
- At 20°C:
- Loss in xylanase activity: 7%
- Loss in glucanase activity: 8%
- At 30°C:
- Loss in xylanase activity: 4%
- Loss in glucanase activity: 2%
Homogeneity
The ability to evenly distribute enzymes in feed was assessed in a study by analysing 10 samples from the stability studies. The results showed the following coefficients of variation:
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For xylanase activity:
- 4.6% in mash diets
- 6.8% in pelleted diets
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For glucanase activity:
- 11.6% in mash diets
- 7.0% in pelleted diets
These coefficients indicate the consistency of enzyme distribution in the different types of feed.
Carrier
Solid form Rovabio Advance
Fermentation concentrate: 15-25%
wheat flour: 75-85%
Liquid form Rovabio Advance:
Fermentation concentrate: 4-8%
Sorbitol: 10-30%
Potassium sorbate: 0.1 - 0.4%
Water: remaining part
Handling
The European Commission states: "For users of the additive and premixtures, feed business operators shall establish operational procedures and organisational measures to address potential risks resulting from its use. Where those risks cannot be eliminated by such procedures and measures, the additive and premixtures shall be used with personal protective equipment, including breathing protection, eye protection and skin protection
Manufacturer
Adisseo
List of references
EFSA (2022). Safety and efficacy of a feed additive consisting of endo-1,4-beta-xylanase and endo-1,3(4)-beta-glucanase produced with Talaromyces versatilis IMI 378536 and DSM 26702 (ROVABIO® ADVANCE) for weaned piglets and pigs for fattening (ADISSEO France S.A.S). EFSA Journal, 2022;20(5):7251
EU (2015). COMMISSION IMPLEMENTING REGULATION (EU) 2015/661 of 28 April 2015 concerning the authorisation of the preparation of endo-1,4-beta-xylanase and endo-1,3(4)-betaglucanase produced by Talaromyces versatilis sp. nov. IMI CC 378536 and Talaromyces versatilis sp. nov. DSM 26702 as a feed additive for chickens for fattening, chickens reared for laying and minor poultry species for fattening and reared for laying (holder of the authorisation Adisseo
France S.A.S.) France S.A.S.). Official Journal of the European Union. L 307/30-33
EU (2015). COMMISSION IMPLEMENTING REGULATION (EU) 2015/2304 of 10 December 2015 concerning the authorisation of a preparation of endo-1,4-beta-xylanase and endo-1,3(4)-beta-glucanase produced by Talaromyces versatilis sp. nov. IMI CC 378536 and Talaromyces versatilis sp. nov DSM 26702 as a feed additive for turkeys for fattening and for breeding (holder of the authorisation Adisseo France S.A.S.). Official Journal of the European Union, L 326/39-42
EU (2017). COMMISSION IMPLEMENTING REGULATION (EU) 2017/210 of 7 February 2017 concerning the authorisation of a preparation of endo-1,4-beta-xylanase and endo-1,3(4)-beta-glucanase produced by Talaromyces versatilis sp. nov. IMI CC 378536 and Talaromyces versatilis sp. nov. DSM 26702 as a feed additive for laying hens (holder of the authorisation Adisseo France S.A.S.). Official Journal of the European Union, L33/19-22